Polev D E, Nosova Iu K, Krukovskaia L L, Baranova A V, Kozlov A P
Mol Biol (Mosk). 2009 Jan-Feb;43(1):97-102.
Using computational methods for analysis of electronic databases we identified a number of human nucleotide sequences expressed predominantly in tumors. We experimentally studied one of the sequences, which is related to the UniGene database cluster Hs.633957 and located near the telomere in the chromosome 7p22.3. All the RNA sequences of the cluster Hs.633957 are non-coding and their role was not described yet, but expression pattern of the locus makes it theoretically and practically interesting. Here we studied expression of the sequence Hs.633957 in various normal and tumor tissues using reverse transcription polymerase chain reaction. Of all the normal adult tissues studied weak expression was only identified in heart and liver. It was also identified in embryonic brain and kidney. Locus Hs.633957 is expressed in tumors of various tissue origin including tumors of lung, intestines, breast, stomach, cervix, lymph nodes and others. Thus the Hs.633957 locus is expressed predominantly in tumors and may be considered a prospective tumor marker.
通过使用计算方法分析电子数据库，我们鉴定出了一些主要在肿瘤中表达的人类核苷酸序列。我们对其中一个与基因数据库簇Hs.633957相关且位于7号染色体短臂2区2带3亚带端粒附近的序列进行了实验研究。Hs.633957簇的所有RNA序列均为非编码序列，其作用尚未阐明，但该基因座的表达模式在理论和实践上都很有趣。在此，我们使用逆转录聚合酶链反应研究了Hs.633957序列在各种正常组织和肿瘤组织中的表达情况。在所研究的所有正常成人组织中，仅在心脏和肝脏中检测到微弱表达。在胚胎脑和肾中也检测到了表达。Hs.633957基因座在各种组织来源的肿瘤中均有表达，包括肺癌、肠癌、乳腺癌、胃癌、宫颈癌、淋巴结癌等。因此，Hs.633957基因座主要在肿瘤中表达，可被视为一种有前景的肿瘤标志物。
