Polev Dmitrii E, Karnaukhova Iuliia K, Krukovskaya Larisa L, Kozlov Andrei P
Biomedical Center, 8 Vyborgskaja Street, Saint Petersburg 194044, Russia ; Department of Genetics and Breeding, Saint Petersburg State University, 7/9 Universitetskaya Embankment, Saint Petersburg 199034, Russia.
Biomedical Center, 8 Vyborgskaja Street, Saint Petersburg 194044, Russia.
Biomed Res Int. 2014;2014:398097. doi: 10.1155/2014/398097. Epub 2014 Feb 24.
Human gene LOC100505644 uncharacterized LOC100505644 [Homo sapiens] (Entrez Gene ID 100505644) is abundantly expressed in tumors but weakly expressed in few normal tissues. Till now the function of this gene remains unknown. Here we identified the chromosomal borders of the transcribed region and the major splice form of the LOC100505644-specific transcript. We characterised the major regulatory motifs of the gene and its splice sites. Analysis of the secondary structure of the major transcript variant revealed a hairpin-like structure characteristic for precursor microRNAs. Comparative genomic analysis of the locus showed that it originated in primates de novo. Taken together, our data indicate that human gene LOC100505644 encodes some non-protein coding RNA, likely a microRNA. It was assigned a gene symbol ELFN1-AS1 (ELFN1 antisense RNA 1 (non-protein coding)). This gene combines features of evolutionary novelty and predominant expression in tumors.
人类基因LOC100505644，未表征的LOC100505644 [智人]（Entrez基因ID 100505644）在肿瘤中大量表达，但在少数正常组织中表达较弱。到目前为止，该基因的功能仍然未知。在此，我们确定了转录区域的染色体边界以及LOC100505644特异性转录本的主要剪接形式。我们对该基因及其剪接位点的主要调控基序进行了表征。主要转录本变体的二级结构分析揭示了前体微小RNA特有的发夹样结构。该基因座的比较基因组分析表明它是灵长类动物中从头起源的。综上所述，我们的数据表明人类基因LOC100505644编码一些非蛋白质编码RNA，可能是一种微小RNA。它被赋予了基因符号ELFN1-AS1（ELFN1反义RNA 1（非蛋白质编码））。该基因兼具进化新颖性和在肿瘤中优势表达的特征。
