朝着同质性促红细胞生成素迈进：基于非 NCL 的 Gln78-Arg166 糖肽结构域的化学合成。
Toward homogeneous erythropoietin: non-NCL-based chemical synthesis of the Gln78-Arg166 glycopeptide domain.
机构信息
Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, New York 10065, USA.
出版信息
J Am Chem Soc. 2009 Apr 22;131(15):5424-31. doi: 10.1021/ja808704m.
Abstract
Single erythropoietin (EPO) glycoforms with defined mature oligosaccharide structures and amino acid sequences are essential to elucidate the molecular mechanisms by which carbohydrates exert various physiological and metabolic functions and to explore the possible links between carbohydrates and the prevention or management of diseases. To demonstrate that it is possible to generate EPO even without recourse to cysteine-based native chemical ligation, a concise synthesis of the partially protected EPO fragment (78-166) bearing fully mature N- and O-glycans is described.
摘要
单一人促红细胞生成素 (EPO) 糖型具有明确的成熟寡糖结构和氨基酸序列，对于阐明碳水化合物发挥各种生理和代谢功能的分子机制以及探索碳水化合物与疾病的预防或治疗之间的可能联系至关重要。为了证明即使不依赖基于半胱氨酸的天然化学连接也有可能生成 EPO，本文描述了带有完全成熟的 N- 和 O-聚糖的部分保护 EPO 片段 (78-166) 的简洁合成。
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