Li Yaohao, Tran Amy H, Danishefsky Samuel J, Tan Zhongping
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China; Department of Biochemistry and BioFrontiers Institute, University of Colorado, Boulder, CO, United States.
Department of Biochemistry and BioFrontiers Institute, University of Colorado, Boulder, CO, United States.
Methods Enzymol. 2019;621:213-229. doi: 10.1016/bs.mie.2019.02.030. Epub 2019 Mar 14.
Recent advances have demonstrated the feasibility and robustness of chemical synthesis for the production of homogeneously glycosylated protein forms (glycoforms). By taking advantage of the unmatchable flexibility and precision provided by chemical synthesis, the quantitative effects of glycosylation were obtained using chemical glycobiology approaches. These findings greatly advanced our fundamental knowledge of glycosylation. More importantly, analysis of these findings has led to the development of glycoengineering guidelines for rationally improving the properties of peptides and proteins. In this chapter, we present the key experimental steps for chemical biology studies of protein glycosylation, with the aim of facilitating and promoting research in this important but significantly underexplored area of biology.
最近的进展已经证明了化学合成用于生产均匀糖基化蛋白质形式（糖型）的可行性和稳健性。通过利用化学合成提供的无与伦比的灵活性和精确性，使用化学糖生物学方法获得了糖基化的定量效应。这些发现极大地推进了我们对糖基化的基础知识。更重要的是，对这些发现的分析导致了合理改善肽和蛋白质性质的糖工程指南的发展。在本章中，我们介绍了蛋白质糖基化化学生物学研究的关键实验步骤，旨在促进和推动这一重要但显著未被充分探索的生物学领域的研究。
