Porzionato A, Macchi V, Parenti A, De Caro R
Section of Anatomy, Department of Human Anatomy and Physiology, University of Padova, Italy.
Histol Histopathol. 2009 Jun;24(6):707-15. doi: 10.14670/HH-24.707.
The aim of the present work was to investigate the occurrence and immunological characteristics of chronic carotid glomitis in opiate addicts. Carotid bodies were sampled at autopsy from 50 subjects who died of heroin intoxication (mean age 28 years), and from 16 young (24 years) and 10 older subjects (66 years) who died of trauma. Sections were stained with haematoxylin-eosin and azan-Mallory, and immunohistochemistry was carried out with anti-CD45, -CD3, -CD8, -CD4, -CD20, -CD68, -CD56. Inflammatory aggregates were not observed in young cases, but were found in 21/50 (42%) opiate cases and in 4/10 (40%) older cases. Infiltrates were mainly located in subcapsular and interlobular positions, and were also found around nerve fibres. Inflammatory aggregates were mainly composed of T suppressor/cytotoxic lymphocytes (50-80%). Monocytic/macrophagic cells and B lymphocytes comprised about 10% and 5-20% of inflammatory cells, respectively. T helper lymphocytes were fewer and only rare Natural Killer cells were found. Chronic carotid glomitis must be included among the autopsy findings of opiate addiction, and may be ascribed to inflammatory reactions to exogenous immunogens or to responses to drug-induced degenerative changes of carotid body components.
本研究的目的是调查阿片类药物成瘾者慢性颈动脉体炎的发生率及免疫特征。从50例死于海洛因中毒的受试者（平均年龄28岁）、16例年轻（24岁）和10例年长（66岁）死于外伤的受试者尸检时采集颈动脉体。切片用苏木精-伊红和偶氮马洛里染色，并进行抗CD45、-CD3、-CD8、-CD4、-CD20、-CD68、-CD56的免疫组织化学检测。年轻病例中未观察到炎性聚集物，但在21/50（42%）的阿片类药物成瘾病例和4/10（40%）的年长病例中发现了炎性聚集物。浸润主要位于被膜下和小叶间，也见于神经纤维周围。炎性聚集物主要由抑制性/细胞毒性T淋巴细胞组成（50-80%）。单核细胞/巨噬细胞和B淋巴细胞分别占炎性细胞的约10%和5-20%。辅助性T淋巴细胞较少，仅发现罕见的自然杀伤细胞。慢性颈动脉体炎必须纳入阿片类药物成瘾的尸检发现中，可能归因于对外源性免疫原的炎症反应或对药物诱导的颈动脉体成分退行性变化的反应。
