Pu Lee L Q, Cui Xiangdong, Li Jihui, Fink Betsy F, Cibull Michael L, Gao Dayong
Division of Plastic Surgery, Department of Surgery, the University of Kentucky, Lexington, KY, USA.
Aesthet Surg J. 2006 Nov-Dec;26(6):653-61. doi: 10.1016/j.asj.2006.10.005.
Successful long-term preservation of adipose tissues may have an important impact on future clinical application of autologous fat transplantation. Our group has recently developed an optimal cryopreservation method for possible long-term preservation of adipose aspirates.
The purpose of this study was to evaluate the fate of previously cryopreserved adipose aspirates after in vivo administration in an established nude mouse model.
Adipose aspirates were collected from a cosmetic lipoplasty of the patient's abdomen after centrifugation. In the fresh control group (n = 20), fresh adipose aspirates were injected into the posterior scalp of a nude mouse. In the optimal cryopreservation group (n = 20), adipose aspirates after the optimal cryopreservation were injected. In the simple cryopreservation group (n = 20), adipose aspirates after the simple cryopreservation were injected. All animals in each group were observed for gross appearance of maintained fat grafts over their posterior scalps for up to 16 weeks. The final volume and weight of maintained fat grafts and their histology were evaluated at the end of the study.
More maintained volume, weight, and fatty tissue structure of injected free grafts were found in the optimal cryopreservation group compared with the simple cryopreservation group, but the results were still less satisfactory than those in the fresh control group.
Based on this in vivo study, we believe that an optimal cryopreservation method developed in our laboratory provides reasonably good long-term preservation of adipose aspirates. However, further studies may still be warranted to refine our method for optimal cryopreservation of adipose tissues.
成功长期保存脂肪组织可能会对自体脂肪移植的未来临床应用产生重要影响。我们团队最近开发了一种最佳冷冻保存方法，用于可能的脂肪抽吸物长期保存。
本研究的目的是在已建立的裸鼠模型中评估先前冷冻保存的脂肪抽吸物在体内给药后的转归。
从患者腹部的美容抽脂术中收集脂肪抽吸物，经离心处理。在新鲜对照组（n = 20）中，将新鲜脂肪抽吸物注射到裸鼠的后头皮。在最佳冷冻保存组（n = 20）中，注射经最佳冷冻保存后的脂肪抽吸物。在简单冷冻保存组（n = 20）中，注射经简单冷冻保存后的脂肪抽吸物。每组中的所有动物均被观察其后头皮上维持的脂肪移植物的大体外观，最长观察16周。在研究结束时评估维持的脂肪移植物的最终体积、重量及其组织学情况。
与简单冷冻保存组相比，最佳冷冻保存组中注射的游离移植物有更多维持的体积、重量和脂肪组织结构，但结果仍不如新鲜对照组。
基于这项体内研究，我们认为我们实验室开发的最佳冷冻保存方法能为脂肪抽吸物提供相当不错的长期保存效果。然而，可能仍需要进一步研究来完善我们的脂肪组织最佳冷冻保存方法。
