Koçak Polen, Ünsal Naz, Canikyan Serli, Kul Yaren, Cohen Steven R, Tiryaki Tunç
Aesthet Surg J Open Forum. 2023 Apr 4;5:ojad026. doi: 10.1093/asjof/ojad026. eCollection 2023.
Effective cryopreservation allows for the long-term storage of living cells or tissues with the possibility of later clinical applications. Unfortunately, no successful investigations on the long-term preservation of adipose aspirates for prospective autologous fat grafting have been conducted.
In this study, we aimed to compare 3 different freezing methods to preserve adipose aspirates obtained from conventional lipoplasty to determine the optimal cryopreservation technique.
To determine the optimal cryopreservation technique, hematoxylin and eosin staining, MTS assay, and Annexin assay were performed on each of the 3 groups plus a fourth control group. Group 1 served as the control, and fat tissue was analyzed immediately after adipose harvesting with no cryopreservation. For experimental Group 2, 15 mL of adipose aspirates were directly frozen at -80°C for up to 2 weeks. For experimental Group 3, 15 mL of adipose aspirates were frozen inside the adi-frosty containing 100% isopropanol and stored at -80°C for up to 2 weeks. For experimental Group 4, 15 mL of adipose aspirates were frozen with freezing solution containing 90% fetal bovine serum (v/v) and 10% dimethyl sulfoxide (v/v).
The results demonstrated that the experimental Group 3 had significantly more live adipocytes and greater cellular function of adipose aspirates than the experimental Groups 2 and 4.
Cryopreservation with adi-frosty containing 100% isopropanol appears to be the best means of cryopreservation of fat.
有效的冷冻保存能够长期储存活细胞或组织，并有可能用于后续的临床应用。遗憾的是，尚未有关于用于前瞻性自体脂肪移植的脂肪抽吸物长期保存的成功研究。
在本研究中，我们旨在比较3种不同的冷冻方法来保存从传统脂肪抽吸术中获得的脂肪抽吸物，以确定最佳的冷冻保存技术。
为确定最佳冷冻保存技术，对3个实验组和1个第四对照组分别进行苏木精和伊红染色、MTS检测及膜联蛋白检测。第1组作为对照组，脂肪组织在采集后立即进行分析，未进行冷冻保存。对于实验组2，将15 mL脂肪抽吸物直接在-80°C冷冻长达2周。对于实验组3，将15 mL脂肪抽吸物在含有100%异丙醇的绝热冷冻器中冷冻，并在-80°C储存长达2周。对于实验组4，将15 mL脂肪抽吸物用含有90%胎牛血清（v/v）和10%二甲基亚砜（v/v）的冷冻溶液冷冻。
结果表明，实验组3的活脂肪细胞明显多于实验组2和4，且脂肪抽吸物的细胞功能更强。
用含有100%异丙醇的绝热冷冻器进行冷冻保存似乎是脂肪冷冻保存的最佳方法。
