苏丹达尔富尔戊型肝炎病毒感染暴发：干血斑实时逆转录聚合酶链反应分析的有效性
Outbreak of hepatitis E virus infection in Darfur, Sudan: effectiveness of real-time reverse transcription-PCR analysis of dried blood spots.
作者信息
Mérens Audrey, Guérin Philippe Jean, Guthmann Jean-Paul, Nicand Elisabeth
机构信息
National Reference Laboratory for Hepatitis E, Hospital Val-de-Grâce, Paris, France.
出版信息
J Clin Microbiol. 2009 Jun;47(6):1931-3. doi: 10.1128/JCM.02245-08. Epub 2009 Apr 1.
Abstract
Biological samples collected in refugee camps during an outbreak of hepatitis E were used to compare the accuracy of hepatitis E virus RNA amplification by real-time reverse transcription-PCR (RT-PCR) for sera and dried blood spots (concordance of 90.6%). Biological profiles (RT-PCR and serology) of asymptomatic individuals were also analyzed.
摘要
在戊型肝炎疫情期间，从难民营收集的生物样本被用于比较实时逆转录聚合酶链反应（RT-PCR）对血清和干血斑进行戊型肝炎病毒RNA扩增的准确性（一致性为90.6%）。还分析了无症状个体的生物学特征（RT-PCR和血清学）。
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