Intorne Aline C, de Oliveira Marcos Vinicius V, Lima Mariana L, da Silva Juliana F, Olivares Fábio L, de Souza Filho Gonçalo Apolinário
Laboratório de Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, RJ, Brazil.
Arch Microbiol. 2009 May;191(5):477-83. doi: 10.1007/s00203-009-0472-0. Epub 2009 Apr 2.
Gluconacetobacter diazotrophicus is a plant-growth-promoting bacterium, which is able to colonize sugarcane and other plant species of economic importance. The potentially beneficial effects promoted by this bacterium on plants are nitrogen-fixation, production of phythormones, action against pathogens and mineral nutrient solubilization. In this study, the molecular mechanisms associated with phosphorus and zinc solubilization were analyzed. A transposon mutant library was constructed and screened to select for mutants defective for phosphorous [Ca(5)(PO(4))(3)OH] and zinc (ZnO) solubilization. A total of five mutants were identified in each screen. Both screenings, performed independently, allowed to select the same mutants. The interrupted gene in each mutant was identified by sequencing and the results demonstrate that the production of gluconic acid is a required pathway for solubilization of such nutrients in G. diazotrophicus.
固氮葡糖醋杆菌是一种促进植物生长的细菌，能够定殖于甘蔗和其他具有经济重要性的植物物种。这种细菌对植物的潜在有益作用包括固氮、植物激素的产生、对抗病原体以及矿质养分的溶解。在本研究中，分析了与磷和锌溶解相关的分子机制。构建并筛选了一个转座子突变体文库，以选择对磷[Ca(5)(PO(4))(3)OH]和锌(ZnO)溶解有缺陷的突变体。在每次筛选中总共鉴定出五个突变体。两次独立进行的筛选都选出了相同的突变体。通过测序鉴定了每个突变体中被中断的基因，结果表明，葡萄糖酸的产生是固氮葡糖醋杆菌溶解此类养分的必要途径。
