Johansson E, Claesson R, van Dijken J W V
Dental Hygienist Education, Department of Odontology, Dental School, Umeå University, Umeå SE-901 87, Sweden.
J Dent. 2009 Jun;37(6):449-53. doi: 10.1016/j.jdent.2009.02.004. Epub 2009 Apr 1.
The aim was to evaluate the antibacterial effect of ozone on cariogenic bacterial species with and without the presence of saliva and a possible effect on the salivary proteins.
Suspensions of Actinomyces naeslundii (ACTCC 12104(T)), Lactobacilli casei (N CTC 151) and Streptococcus mutans (NCTC 10449), in salt buffer or in saliva, were exposed to ozone gas delivered by the ozone generator Healozone 2130C. Aliquots of the suspensions were taken after 10, 30 and 60s ozone exposures and cultivated on agar plates. Initial number of bacteria per ml was 8.0 x 10(7) (SD 2.2 x 10(7)) (A. naeslundii), 1.0 x 10(8) (SD 3.1 x 10(6)) (L. casei) and 1.0 x 10(8) (SD 7.0 x 10(5)) (S. mutans), respectively. The proteins were separated by SDS electrophoresis and visualized by silver staining.
In salt buffer 92%, 73% and 64% of the initial numbers of A. naeslundii, S. mutans and L. casei, respectively, were killed already after 10s ozone exposure, while approximately 99.9% of the bacteria were dead after a 60s exposure. After 10 and 30s, but not after 60s exposure to ozone, S. mutans and L. casei were less efficiently killed in saliva compared to the salt buffer. Various saliva proteins were degraded by ozone after a 60s exposure.
The cariogenic species S. mutans, L. casei and A. naeslundii were almost eliminated following 60s of ozone treatment. This killing was reduced in the presence of saliva although increasing the ozone application time to 60s overcame these reductants in saliva. Detection of altered salivary proteins indicates that saliva components constitute additional targets for ozone.
评估臭氧在有或无唾液存在的情况下对致龋细菌的抗菌效果，以及对唾液蛋白的可能影响。
将内氏放线菌（ACTCC 12104(T)）、干酪乳杆菌（NCTC 151）和变形链球菌（NCTC 10449）分别悬浮于盐缓冲液或唾液中，然后暴露于臭氧发生器Healozone 2130C产生的臭氧气体中。在臭氧暴露10秒、30秒和60秒后，取悬浮液的等分试样接种于琼脂平板上培养。每毫升细菌的初始数量分别为8.0×10⁷（标准差2.2×10⁷）（内氏放线菌）、1.0×10⁸（标准差3.1×10⁶）（干酪乳杆菌）和1.0×10⁸（标准差7.0×10⁵）（变形链球菌）。通过SDS电泳分离蛋白质，并用银染法进行可视化。
在盐缓冲液中，臭氧暴露10秒后，内氏放线菌、变形链球菌和干酪乳杆菌初始数量的92%、73%和64%已被杀死，而暴露60秒后约99.9%的细菌死亡。暴露10秒和30秒后，但不是60秒后，与盐缓冲液相比，变形链球菌和干酪乳杆菌在唾液中的杀灭效率较低。暴露60秒后，各种唾液蛋白被臭氧降解。
致龋菌变形链球菌、干酪乳杆菌和内氏放线菌在臭氧处理60秒后几乎被消除。在有唾液存在的情况下，这种杀灭作用有所降低，尽管将臭氧应用时间延长至60秒可克服唾液中的这些还原剂。唾液蛋白变化的检测表明，唾液成分是臭氧的额外作用靶点。
