Translational and Experimental Clinical Research Centre in Oral Health, University of Medicine and Pharmacy "Victor Babes", Eftimie Murgu Sq. no 2, 300041 Timisoara, Romania.
Department of Preventive, Community Dentistry and Oral Health, University of Medicine and Pharmacy "Victor Babes", Eftimie Murgu Sq. no 2, 300041 Timisoara, Romania.
Molecules. 2023 Feb 21;28(5):2032. doi: 10.3390/molecules28052032.
This study aimed to obtain and analyse essential oil (MpEO) for the prospect of being used as an enhancement agent for the antimicrobial potential of ozone against gram-positive and gram-negative bacteria and fungi. The research was done for different exposure times, and it gained time-dose relationships and time-effect correlations. (Mp) essential oil (MpEO) was obtained via hydrodistillation and further analysed by using GC-MS. The broth microdilution assay was used to determine the strain inhibition/strain mass growth by using spectrophotometric optical density reading (OD). The bacterial/mycelium growth rates (BGR/MGR) and the bacterial/mycelium inhibition rates (BIR/MIR) after ozone treatment in the presence and absence of MpEO on the ATTC strains were calculated; the minimum inhibition concentration (MIC) and statistical interpretations of the time-dose relationship and specific -test correlations were determined. The effect of ozone on the following tested strains at maximum efficiency was observed after 55 s of single ozone exposure, in order of effect strength: > > > > . For ozone with the addition of 2% MpEO (MIC), maximum efficacy was recorded at 5 s for these strains, in order of effect strength: > > > > . The results suggest a new development and affinity regarding the cell membrane of the different microorganisms tested. In conclusion, the use of ozone, combined with MpEO, is sustained as an alternative therapy in plaque biofilm and suggested as helpful in controlling oral disease-causing microorganisms in medicine.
本研究旨在获得和分析精油（MpEO），以期将其作为增强臭氧对革兰氏阳性和革兰氏阴性细菌及真菌的抗菌潜力的添加剂。研究针对不同的暴露时间进行，以获得时间-剂量关系和时间-效应相关性。通过水蒸馏获得（Mp）精油（MpEO），并进一步通过 GC-MS 进行分析。使用肉汤微量稀释法通过分光光度光密度读数（OD）来确定菌株抑制/菌株质量生长。在存在和不存在 MpEO 的情况下，计算臭氧处理后 ATTC 菌株的细菌/菌丝生长率（BGR/MGR）和细菌/菌丝抑制率（BIR/MIR）；确定最小抑制浓度（MIC）和时间-剂量关系以及特定检验相关性的统计解释。观察到臭氧对以下经测试菌株的影响在单一臭氧暴露 55 秒后达到最大效率，按效果强度顺序为： > > > > > 。对于添加 2% MpEO（MIC）的臭氧，这些菌株的最大功效记录在 5 秒时，按效果强度顺序为： > > > > > 。结果表明，针对不同测试微生物的细胞膜有新的发展和亲和力。总之，臭氧与 MpEO 联合使用被认为是一种斑块生物膜的替代疗法，并被建议有助于控制医学中引起口腔疾病的微生物。
