Treichel Helen, Mazutti Marcio Antonio, Maugeri Francisco, Rodrigues Maria Isabel
Department of Food Engineering, URI, Campus de Erechim, P.O. Box 743, Erechim, RS, CEP 99700-000, Brazil.
J Ind Microbiol Biotechnol. 2009 Jul;36(7):895-900. doi: 10.1007/s10295-009-0567-2. Epub 2009 Apr 3.
A potential application of inulinase in the food industry is the production of fructoligosaccharides (FOS) by the transfructosilation of sucrose. The FOSs present many interesting functional properties besides their ability to increase the shelf-life and flavor of many products. The use of an industrial medium represents a good alternative to producing inulinase at low cost, since the activity may improve, or at least remain the same, as that obtained using a synthetic medium. This work was an optimization study of the inulinase production by Kluyveromyces marxianus NRRL Y-7571 using industrial pre-treated culture medium in a bioreactor employing a sequential strategy of experimental design. Initially, a Plackett-Burman (Screening Design) design was used, where the studied variables were molasses, corn steep liquor, yeast extract concentration, and agitation and aeration rates. After the analysis of the effects, a central composite rotational design (CCRD) was carried out. The optimized condition for the inulinase production was: 250 g/l of molasses, 80 g/l of corn steep liquor, 6 g/l of yeast extract, 300 rpm of agitation and 1.5 vvm aeration rate, which resulted in an enzymatic activity of 1,317 +/- 65 U/ml.
菊粉酶在食品工业中的一个潜在应用是通过蔗糖的转果糖基化作用生产低聚果糖（FOS）。除了能够延长许多产品的保质期和改善风味外，低聚果糖还具有许多有趣的功能特性。使用工业培养基是低成本生产菊粉酶的一个很好的替代方法，因为其活性可能会提高，或者至少与使用合成培养基所获得的活性相同。这项工作是对马克斯克鲁维酵母NRRL Y - 7571在生物反应器中使用工业预处理培养基生产菊粉酶进行的优化研究，采用了顺序实验设计策略。最初，使用了Plackett - Burman（筛选设计），其中研究的变量是糖蜜、玉米浆、酵母提取物浓度以及搅拌和通气速率。在分析了这些影响之后，进行了中心复合旋转设计（CCRD）。菊粉酶生产的优化条件为：250 g/l糖蜜、80 g/l玉米浆、6 g/l酵母提取物、300 rpm搅拌速度和1.5 vvm通气速率，这导致酶活性为1317±65 U/ml。
