Shimojo N, Fujino K, Kitahashi S, Nakao M, Naka K, Okuda K
Department of Laboratory Medicine, Osaka City University Medical School, Japan.
Clin Chem. 1991 Nov;37(11):1978-80.
To monitor changes in the concentration of blood lactate during physical exercise, we used an automated lactate analyzer based on an electro-enzymatic method with continuous blood sampling through a catheter. The lactate concentration was measured every 2 min; between measurements, the instrument was calibrated with a lactate standard. Ascorbic acid, bilirubin, hemoglobin, creatinine, uric acid, and glucose did not interfere with the measurements. The lactate concentrations in blood samples from apparently healthy subjects before and after exercise correlated well (r = 0.993) with results by the conventional enzymatic method. We measured the blood lactate concentrations in nine apparently healthy volunteers during exercise on a treadmill with an increasing workload. The point at which lactate concentrations started to increase was detected easily. Thus, the lactate analyzer is suitable for monitoring changes in blood lactate concentrations during exercise.
为监测体育锻炼期间血乳酸浓度的变化，我们使用了一台基于电酶法的自动乳酸分析仪，通过导管进行连续血样采集。每2分钟测量一次乳酸浓度；在两次测量之间，用乳酸标准品对仪器进行校准。抗坏血酸、胆红素、血红蛋白、肌酐、尿酸和葡萄糖均不干扰测量。运动前后来自明显健康受试者的血样中的乳酸浓度与传统酶法的结果具有良好的相关性（r = 0.993）。我们在跑步机上对九名明显健康的志愿者进行了递增负荷运动，期间测量了他们的血乳酸浓度。乳酸浓度开始升高的点很容易被检测到。因此，该乳酸分析仪适用于监测运动期间血乳酸浓度的变化。
