Weil M H, Leavy J A, Rackow E C, Halfman C J, Bruno S J
Clin Chem. 1986 Dec;32(12):2175-7.
An electrode-based lactate analyzer was recently developed to facilitate "stat" laboratory measurement of lactate in whole blood. The results obtained with this technique were compared with those of our continuous-flow enzymatic method for 172 analyses of samples from 25 pigs; 88 analyses of samples from 12 normal human volunteers before, during, and after exercise; and 91 analyses of samples from 80 critically ill patients. This comparison revealed strongly significant linear correlations: r = 0.992 for the porcine analyses and r = 0.994 for the combined human analyses. In analytical-recovery studies with pooled porcine plasma with an initial lactate concentration of 3.85 mmol/L, 94.3% of added lactate (1, 5, and 10 mmol/L) was accounted for in the electrode technique, 96% in the reference technique. We conclude that the electrode-based lactate analyzer is rapid, precise, and accurate for measurement of lactate in whole-blood samples.
最近研发了一种基于电极的乳酸分析仪，以方便在“急诊”实验室中对全血中的乳酸进行测量。将该技术获得的结果与我们的连续流动酶法的结果进行了比较，共对25头猪的样本进行了172次分析；对12名正常人类志愿者在运动前、运动期间和运动后的样本进行了88次分析；对80名重症患者的样本进行了91次分析。这种比较显示出极强的线性相关性：猪样本分析的r = 0.992，人类样本综合分析的r = 0.994。在对初始乳酸浓度为3.85 mmol/L的混合猪血浆进行的分析回收率研究中，电极技术中添加的乳酸（1、5和10 mmol/L）有94.3%得到回收，参考技术中为96%。我们得出结论，基于电极的乳酸分析仪在测量全血样本中的乳酸时快速、精确且准确。
