Jensen H S, Jensen L T, Saxne T, Diamant M, Bendtzen K
Department of Rheumatology 232, Hvidorve University Hospital, Denmark.
Clin Exp Rheumatol. 1991 Jul-Aug;9(4):391-4.
Elastolytic activity by live human monocytes (M phi) is mainly caused by cell surface related leucocyte elastase, capable of degrading matrix components. In order to examine the possible correlation between enzyme activity and tissue turnover in the joint, we examined 24 synovial fluids for M phi elastolytic activity, using the levels of synovial fluid interleukin-6 and serum C reactive protein as additional markers of cell activation. Proteoglycan levels were measured as an indication of cartilage degradation and the types I and III procollagen propeptides as markers of synovial membrane turnover. We found that elastolysis by live M phi and the levels of interleukin-6 and C reactive protein correlated significantly with proteoglycan concentrations but not with the procollagen propeptides. These findings suggest that human M phi elastolytic activation is a biologically relevant factor in cartilage degradation, but is unrelated to the collagen metabolism of the synovial membrane.
活的人单核细胞（M phi）的弹性蛋白酶活性主要由与细胞表面相关的白细胞弹性蛋白酶引起，该酶能够降解基质成分。为了研究酶活性与关节组织更新之间的可能相关性，我们检测了24份滑液中的M phi弹性蛋白酶活性，并将滑液白细胞介素-6水平和血清C反应蛋白作为细胞活化的附加标志物。测量蛋白聚糖水平以指示软骨降解情况，并将I型和III型前胶原前肽作为滑膜更新的标志物。我们发现，活的M phi的弹性蛋白酶解作用以及白细胞介素-6和C反应蛋白水平与蛋白聚糖浓度显著相关，但与前胶原前肽无关。这些发现表明，人M phi弹性蛋白酶解活化是软骨降解中的一个生物学相关因素，但与滑膜的胶原代谢无关。
