Saxne T, Heinegård D
Department of Rheumatology, Lund University, Sweden.
Arthritis Rheum. 1992 Apr;35(4):385-90. doi: 10.1002/art.1780350404.
To investigate whether fragmentation of proteoglycans in arthritis results in domains that have different levels of release from cartilage at different stages of the disease.
Two regions of the proteoglycan, the hyaluronan-binding region and the glycosaminoglycan-rich region of the core protein, were measured, by immunoassay, in knee joint synovial fluids of patients with rheumatoid arthritis or reactive arthritis.
Synovial fluid concentrations of the glycosaminoglycan-rich region were highest in rheumatoid arthritis patients who had little cartilage damage as determined by radiography, whereas release of the hyaluronan-binding region predominated in patients with advanced cartilage destruction. In reactive arthritis, release of the glycosaminoglycan-rich region predominated.
These findings indicate that the hyaluronan-binding region is initially retained in the tissue during the development of cartilage destruction. The combined analysis of these markers offers a new avenue for assessment of the degree of cartilage damage in arthritis.
研究关节炎中蛋白聚糖的片段化是否会导致在疾病不同阶段从软骨释放水平不同的结构域。
通过免疫测定法，对类风湿性关节炎或反应性关节炎患者膝关节滑液中蛋白聚糖的两个区域，即透明质酸结合区域和核心蛋白的富含糖胺聚糖区域进行测量。
通过X线摄影确定软骨损伤较小的类风湿性关节炎患者，其富含糖胺聚糖区域的滑液浓度最高，而在软骨严重破坏的患者中，透明质酸结合区域的释放占主导。在反应性关节炎中，富含糖胺聚糖区域的释放占主导。
这些发现表明，在软骨破坏发展过程中，透明质酸结合区域最初保留在组织中。对这些标志物的综合分析为评估关节炎中软骨损伤程度提供了一条新途径。
