Saxne T, Di Giovine F S, Heinegård D, Duff G W, Wollheim F A
Department of Rheumatology, Lund University Hospital, Sweden.
J Autoimmun. 1988 Aug;1(4):373-80. doi: 10.1016/0896-8411(88)90007-8.
Interleukin-1 beta (IL-1 beta) and proteoglycans have been quantified by radioimmunoassay (IL-1 beta) and enzyme linked immunosorbent assay (proteoglycans) in synovial fluids and sera from patients with rheumatoid arthritis (RA) and reactive arthritis. All fluids were also tested for their ability to influence proteoglycan metabolism in a cartilage explant culture system. Synovial fluid IL-1 beta concentrations were inversely related to proteoglycan concentrations in samples from both RA and reactive arthritis patients (P less than 0.002 for all patients). There was no statistically significant relation between immunoreactive IL-1 beta concentration and proteoglycan synthesis or degradation in explants cultured in synovial fluid containing medium. Synovial fluid IL-1 beta concentrations were not related to erythrocyte sedimentation rate or joint total leukocyte count. IL-1 beta was not detectable (limit 250 pg/ml) in any unextracted sera. Although it appears likely that IL-1 beta is involved in the inflammatory and degenerative processes in joint disease, our findings indicate that there is no simple positive relationship between immunoreactive levels of this cytokine in synovial fluid and liberation of proteoglycans from articular cartilage as reflected in synovial fluid proteoglycan concentration.
采用放射免疫分析法（检测白细胞介素-1β，IL-1β）和酶联免疫吸附测定法（检测蛋白聚糖）对类风湿性关节炎（RA）和反应性关节炎患者的滑液和血清中的白细胞介素-1β和蛋白聚糖进行了定量分析。所有滑液还在软骨外植体培养系统中检测了其影响蛋白聚糖代谢的能力。RA患者和反应性关节炎患者样本中，滑液白细胞介素-1β浓度与蛋白聚糖浓度呈负相关（所有患者P<0.002）。在含有滑液的培养基中培养的外植体中，免疫反应性白细胞介素-1β浓度与蛋白聚糖合成或降解之间无统计学显著关系。滑液白细胞介素-1β浓度与红细胞沉降率或关节总白细胞计数无关。在任何未提取的血清中均未检测到白细胞介素-1β（检测限为250 pg/ml）。尽管白细胞介素-1β似乎参与了关节疾病的炎症和退行性过程，但我们的研究结果表明，滑液中这种细胞因子的免疫反应水平与滑液蛋白聚糖浓度所反映的关节软骨蛋白聚糖释放之间不存在简单的正相关关系。
