Saxne T, Heinegård D, Wollheim F A
Department of Rheumatology, University Hospital, Lund, Sweden.
Coll Relat Res. 1988 May;8(3):233-47. doi: 10.1016/s0174-173x(88)80043-8.
The influence of synovial fluid and serum from patients with inflammatory joint disease on proteoglycan metabolism was studied in organ culture of bovine nasal cartilage. Proteoglycan biosynthesis, i.e. incorporation of [35S]-sulphate, was reduced after addition of synovial fluid from rheumatoid arthritis and reactive arthritis patients. Also some rheumatoid arthritis sera but no reactive arthritis serum reduced the biosynthesis compared to control sera. Proteoglycan degradation, i.e. release of proteoglycans prelabelled with [35S]-sulphate, as well as release of proteoglycans determined by chemical methods, was highest under the influence of rheumatoid arthritis synovial fluid. This effect appears to represent an activity truly stimulating degradation, since added control serum did not prevent the effect. The lowest proteoglycan degradation was observed in culture medium only. Addition of synovial fluid compared to addition of control serum did not increase proteoglycan degradation in freeze-killed cartilage indicating that the effect requires living cells. The findings are consistent with the presence in synovial fluid of mediators stimulating the chondrocytes both to activate proteoglycan degradation and to reduce proteoglycan biosynthesis.
在牛鼻软骨器官培养中研究了炎性关节病患者的滑液和血清对蛋白聚糖代谢的影响。添加类风湿性关节炎和反应性关节炎患者的滑液后，蛋白聚糖生物合成（即[35S] - 硫酸盐的掺入）减少。与对照血清相比，一些类风湿性关节炎血清也会降低生物合成，但反应性关节炎血清不会。蛋白聚糖降解，即预先用[35S] - 硫酸盐标记的蛋白聚糖的释放，以及通过化学方法测定的蛋白聚糖的释放，在类风湿性关节炎滑液的影响下最高。这种作用似乎代表了一种真正刺激降解的活性，因为添加对照血清并不能阻止这种作用。仅在培养基中观察到最低的蛋白聚糖降解。与添加对照血清相比，添加滑液并没有增加冻存软骨中的蛋白聚糖降解，这表明这种作用需要活细胞。这些发现与滑液中存在刺激软骨细胞激活蛋白聚糖降解并减少蛋白聚糖生物合成的介质一致。
