Mortellaro Alessandra, Urbano Matteo, Citterio Stefania, Foti Maria, Granucci Francesca, Ricciardi-Castagnoli Paola
Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore.
Methods Mol Biol. 2009;531:17-27. doi: 10.1007/978-1-59745-396-7_2.
Substantial progress has been made over the last several years in the development of protocols for the isolation of large numbers of dendritic cells (DCs) from different tissues and their short-term culture. Indeed, several stable DC lines and clones have been established from various tissues of mice and humans, providing useful experimental tools for studying the biology of DCs at both molecular and biochemical levels and for the establishment of new DC-based immunotherapies. In this chapter, we will describe the development of long-term DC lines that maintain the growth factor dependence and their immature functional state, thus providing a unique opportunity to study the mechanisms of the initiation of the immune response to infectious agents.
在过去几年中，从不同组织中分离大量树突状细胞（DCs）并进行短期培养的方案开发取得了重大进展。事实上，已经从小鼠和人类的各种组织中建立了几种稳定的DC系和克隆，为在分子和生化水平上研究DCs的生物学特性以及建立新的基于DC的免疫疗法提供了有用的实验工具。在本章中，我们将描述长期DC系的开发，这些细胞系保持生长因子依赖性及其未成熟的功能状态，从而为研究针对感染因子的免疫反应启动机制提供了独特的机会。
