Hofmann W W
Arch Neurol. 1977 Jun;34(6):356-60. doi: 10.1001/archneur.1977.00500180050010.
Tests were made in vitro fo the action of prednisolone on nerve backfiring, muscle twitches, tetanus decay rates, miniature end-plate potential amplitude and frequency, and the block induced by curare, hemicholinium, and excess magnesium. At about 0.1 mM concentration, the steroid showed no 'veratrinic' or decurarizing action, and the probability of transmitter release was not increased in 10mM Mg++. Moreover, when acetylcholine stores were depleted in hemicholinium, there was no evidence of repair by steroid. The results are taken to mean that prednisolone and congeners do not owe their therapeutic efficacy in myasthenia to actions at the neuromuscular junction. It is therefore concluded that the clinical benefits from steroids are related to systemic, possibly immunosuppressive effects.
对泼尼松龙在体外对神经逆向放电、肌肉抽搐、破伤风衰减率、微小终板电位幅度和频率以及箭毒、半胆碱和过量镁诱导的阻滞作用进行了测试。在约0.1 mM浓度下，该类固醇未显示出“藜芦碱样”或箭毒碱样作用，且在10 mM Mg++中递质释放概率未增加。此外，当半胆碱使乙酰胆碱储存耗尽时，没有证据表明类固醇能进行修复。这些结果意味着泼尼松龙及其同系物在重症肌无力中的治疗效果并非归因于其在神经肌肉接头处的作用。因此得出结论，类固醇的临床益处与全身作用有关，可能是免疫抑制作用。
