James G T, Yeoman L C, Matsui S i, Goldberg A H, Busch H
Biochemistry. 1977 May 31;16(11):2384-9. doi: 10.1021/bi00630a012.
The nonhistone chromatin protein, C-14, was extracted from chromatin of Novikoff hepatoma ascites cells and isolated in high purity as shown by its migration as a single dense spot on two-dimensional polyacrylamide gels. Its mobility on sodium dodecyl sulfate gels is consistent with a molecular weight of approximately 70 000. The amino acid composition shows that protein C-14 has an acidic:basic amino acid ratio of 1.8. Its amino terminal amino acid is lysine. Protein C-14 stimulated the incorporation of [3H]UMP into RNA by approximately 30% when added to naked DNA and homologous RNA polymerase I. A 30% stimulation of [3H]UMP incorporation into RNA was also found when protein C-14 was added to an E. coli RNA polymerase system containing either E. coli or Novikoff hepatoma DNA.
非组蛋白染色质蛋白C - 14是从诺维科夫肝癌腹水细胞的染色质中提取的，并以高纯度分离出来，这在二维聚丙烯酰胺凝胶上表现为单一密集斑点的迁移。它在十二烷基硫酸钠凝胶上的迁移率与分子量约为70000一致。氨基酸组成表明蛋白C - 14的酸性氨基酸与碱性氨基酸的比例为1.8。其氨基末端氨基酸是赖氨酸。当将蛋白C - 14添加到裸露的DNA和同源RNA聚合酶I中时，它能使[³H]UMP掺入RNA的量增加约30%。当将蛋白C - 14添加到含有大肠杆菌或诺维科夫肝癌DNA的大肠杆菌RNA聚合酶系统中时，也发现[³H]UMP掺入RNA的量有30%的增加。
