通过二维聚丙烯酰胺凝胶电泳比较正常大鼠肝脏和诺维科夫肝癌腹水细胞的核仁蛋白。
Comparison of nucleolar proteins of normal rat liver and Novikoff hepatoma ascites cells by two-dimensional polyacrylamide gel electrophoresis.
作者信息
Orrick L R, Olson M O, Busch H
出版信息
Proc Natl Acad Sci U S A. 1973 May;70(5):1316-20. doi: 10.1073/pnas.70.5.1316.
A two-dimensional polyacrylamide gel electrophoresis technique is presented for separation of acid-extracted proteins of isolated nucleoli of normal rat liver and Novikoff hepatoma ascites cells. About 100 distinct protein spots were resolved. Comparison of the patterns for normal liver and Novikoff hepatoma revealed nine spots with markedly different intensities in the two patterns. Two spots were found that were unique to the normal liver pattern, and one spot was found that was unique to the hepatoma pattern.
本文介绍了一种二维聚丙烯酰胺凝胶电泳技术，用于分离正常大鼠肝脏和诺维科夫肝癌腹水细胞分离核仁的酸提取蛋白。大约分辨出100个不同的蛋白斑点。正常肝脏和诺维科夫肝癌模式的比较显示，两种模式中有9个斑点的强度明显不同。发现两个斑点是正常肝脏模式所特有的，一个斑点是肝癌模式所特有的。
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