用于模拟核糖体反应的底物的设计与合成。

Design and synthesis of substrates for model ribosomal reactions.

作者信息

Bayryamov Stanislav G, Vassilev Nikolay G, Rangelov Miroslav A, Mladjova Aneta P, Petkov Dimiter D

机构信息

Laboratory of BioCatalysis, Institute of Organic Chemistry, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria.

出版信息

Protein Pept Lett. 2009;16(4):392-401. doi: 10.2174/092986609787848009.

DOI:10.2174/092986609787848009

PMID:19356136

Abstract

2'/3'-O-[Bz(NO(2))-Orn(Boc)]-5'-O-Piv-Ado (1) and its deoxy analog: 3'-O-[Bz(NO(2))-Orn(Boc)]-5'-O-Piv-2'-dAdo (2) were designed and synthesized as substrates for the model ribosome reaction we used to demonstrate the crucial role of A76 2'-OH of peptidyl-tRNA in the rate acceleration of peptide bond formation during protein biosynthesis.

摘要

2'/3'-O-[二苯甲酰亚胺基（硝基）-鸟氨酸（叔丁氧羰基）]-5'-O-新戊酰基-腺苷（1）及其脱氧类似物：3'-O-[二苯甲酰亚胺基（硝基）-鸟氨酸（叔丁氧羰基）]-5'-O-新戊酰基-2'-脱氧腺苷（2）被设计并合成作为模型核糖体反应的底物，我们用该反应来证明肽基-tRNA的A76 2'-羟基在蛋白质生物合成过程中肽键形成速率加速中的关键作用。