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酵母40 kDa蛋白的酪氨酸磷酸化会因交配信息素而发生。

Tyrosine phosphorylation of a yeast 40 kDa protein occurs in response to mating pheromone.

作者信息

Ballard M J, Tyndall W A, Shingle J M, Hall D J, Winter E

机构信息

Department of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19107.

出版信息

EMBO J. 1991 Dec;10(12):3753-8. doi: 10.1002/j.1460-2075.1991.tb04944.x.

DOI:10.1002/j.1460-2075.1991.tb04944.x
PMID:1935898
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC453111/
Abstract

Tyrosine phosphorylation of proteins in the yeast Saccharomyces cerevisiae has been examined following exposure to the mating pheromone alpha-factor. When a cells are treated with alpha-factor a protein of approximately 40 kDa molecular weight is tyrosine phosphorylated. This tyrosine phosphorylation response requires an intact signal transduction pathway, is not restricted to a short interval of the cell division cycle, and requires protein synthesis for its maximal accumulation. Mating competent fus3 deletion strains fail to elaborate the phosphotyrosine response. The possibility that FUS3 encodes the 40 kDa protein is discussed.

摘要

在酿酒酵母暴露于交配信息素α-因子后，对其蛋白质的酪氨酸磷酸化进行了检测。当α细胞用α-因子处理时，一种分子量约为40 kDa的蛋白质会发生酪氨酸磷酸化。这种酪氨酸磷酸化反应需要完整的信号转导途径，不限于细胞分裂周期的短时间间隔，并且其最大积累需要蛋白质合成。具有交配能力的fus3缺失菌株无法产生磷酸酪氨酸反应。文中讨论了FUS3编码40 kDa蛋白质的可能性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3bc0/453111/b27ef2339971/emboj00110-0192-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3bc0/453111/30674d049fa5/emboj00110-0189-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3bc0/453111/1a5ab3030223/emboj00110-0189-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3bc0/453111/04ba011a35d7/emboj00110-0190-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3bc0/453111/281753852183/emboj00110-0190-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3bc0/453111/c10979bf60b2/emboj00110-0191-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3bc0/453111/5ec35441f5b0/emboj00110-0191-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3bc0/453111/b27ef2339971/emboj00110-0192-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3bc0/453111/30674d049fa5/emboj00110-0189-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3bc0/453111/1a5ab3030223/emboj00110-0189-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3bc0/453111/04ba011a35d7/emboj00110-0190-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3bc0/453111/281753852183/emboj00110-0190-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3bc0/453111/c10979bf60b2/emboj00110-0191-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3bc0/453111/5ec35441f5b0/emboj00110-0191-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3bc0/453111/b27ef2339971/emboj00110-0192-a.jpg

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本文引用的文献

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Mutants of Saccharomyces cerevisiae unresponsive to cell division control by polypeptide mating hormone.对多肽交配激素的细胞分裂控制无反应的酿酒酵母突变体。
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