Portyanko A, Kovalev P, Gorgun J, Cherstvoy E
Department of Pathology, Belarusian State Medical University, 220116, Dzerzhynsky Ave. 83, Minsk, Belarus.
Virchows Arch. 2009 May;454(5):541-8. doi: 10.1007/s00428-009-0764-4. Epub 2009 Apr 10.
Cell locomotion, including cancer cell invasion, is closely associated with the dynamics of cytoskeletal structures. Previous in vitro studies indicated that tubulin isotype composition may affect polymerization properties and dynamics of microtubules. Colorectal cancer is a good model for studying tumour invasion because of the easily detectable invasive front. Hence, we investigated the localization of beta(III)-tubulin in colorectal cancer specimens. Immunohistochemical staining for beta(III)-tubulin was evident in cancer cells apparently budding from adjacent malignant cells with a higher differentiation and negative staining. An association between beta(III)-tubulin immunoreactivity and tumour budding grade was demonstrated. To the best of our knowledge, this is the first report documenting a preferential localization of beta(III)-tubulin in the invading epithelium. From this finding arises the possibility that changes in tubulin isotypes could modulate the invading activity of cancer cells. Further investigations are needed to determine whether our findings have clinical implications.
细胞运动，包括癌细胞侵袭，与细胞骨架结构的动力学密切相关。先前的体外研究表明，微管蛋白亚型组成可能会影响微管的聚合特性和动力学。由于侵袭前沿易于检测，结直肠癌是研究肿瘤侵袭的良好模型。因此，我们研究了β(III)-微管蛋白在结直肠癌标本中的定位。β(III)-微管蛋白的免疫组织化学染色在明显从相邻高分化恶性细胞出芽的癌细胞中明显，且染色为阴性。β(III)-微管蛋白免疫反应性与肿瘤出芽分级之间存在关联。据我们所知，这是第一份记录β(III)-微管蛋白在侵袭性上皮细胞中优先定位的报告。基于这一发现，微管蛋白亚型的变化可能调节癌细胞侵袭活性的可能性由此产生。需要进一步研究以确定我们的发现是否具有临床意义。
