dos Santos Isabele Barbieri, Schubach Tânia M P, Leme Luiz R P, Okamoto Thais, Figueiredo Fabiano B, Pereira Sandro A, Quintella Leonardo P, de F Madeira Maria, dos S Coelho Flávia, Reis Rosani, de O Schubach Armando
Serviço de Zoonoses, Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Av Brasil 4365, 21045-900 Rio de Janeiro, Brazil.
Vet Parasitol. 2007 Jan 19;143(1):1-6. doi: 10.1016/j.vetpar.2006.08.002. Epub 2006 Sep 12.
Seventy-four dogs from the State of Rio de Janeiro with ulcerated cutaneous lesions were submitted to clinical, dermatological, parasitological, mycological, histopathological and cytopathological exams, a leishmanin skin test, an indirect immunofluorescence (IIF) test for leishmaniosis, and nonspecific laboratory tests such as blood count and serum biochemistry. Sporothrix schenckii was isolated from 41 dogs and Leishmania (Viannia) braziliensis was isolated from 33 animals. Most dogs with sporotrichosis were from the municipality of Rio de Janeiro (53.7%) and presented ulcerated cutaneous lesions on the head (68.3%). Laboratory alterations in these animals included anemia (58.5%), hypoalbuminemia (83%) and hyperglobulinemia (75.6%). Histopathology revealed the predominance of a chronic granulomatous inflammatory infiltrate (70.7%), and yeast-like structures were detected in 17% of the histopathological exams and in 32% of the cytological exams. Three of 41 dogs with sporotrichosis were seropositive by IIF for leishmaniosis and 2 of 20 animals tested within this group had a positive leishmanin skin test. Similarly, most of the 33 dogs with leishmaniosis were from the municipality of Rio de Janeiro (69.7%) and had ulcerated cutaneous lesions on the head (84.8%). Laboratory alterations in these animals included anemia (66.7%), hypoalbuminemia (100%) and hyperglobulinemia (91%). Histopathology showed the predominance of a chronic granulomatous inflammatory infiltrate (63.6%) and amastigote forms were detected in 30.3% of the histopathological exams and in 31.8% of the 22 cytological exams performed. About 72.7% of the dogs were seropositive by IIF and five of seven animals had a positive skin test. Due to the clinical similarities, histopathological and nonspecific laboratory results similarities, the serological and skin tests for leishmaniosis positive in dogs with sporotrichosis, and the overlapping endemic areas in Rio de Janeiro, the differential diagnosis between the two diseases requires the demonstration of their respective etiological agents.
对来自里约热内卢州的74只患有皮肤溃疡病变的犬进行了临床、皮肤病学、寄生虫学、真菌学、组织病理学和细胞病理学检查、利什曼原虫皮肤试验、利什曼病间接免疫荧光（IIF）试验以及血常规和血清生化等非特异性实验室检查。从41只犬中分离出申克孢子丝菌，从33只动物中分离出巴西利什曼原虫（维安尼亚利什曼原虫）。大多数患有孢子丝菌病的犬来自里约热内卢市（53.7%），头部出现皮肤溃疡病变（68.3%）。这些动物的实验室检查异常包括贫血（58.5%）、低白蛋白血症（83%）和高球蛋白血症（75.6%）。组织病理学显示以慢性肉芽肿性炎症浸润为主（70.7%），在17%的组织病理学检查和32%的细胞学检查中检测到酵母样结构。41只患有孢子丝菌病的犬中有3只IIF检测利什曼病血清学阳性，该组中检测的20只动物中有2只利什曼原虫皮肤试验呈阳性。同样，33只患有利什曼病的犬中大多数来自里约热内卢市（69.7%），头部有皮肤溃疡病变（84.8%）。这些动物的实验室检查异常包括贫血（66.7%）、低白蛋白血症（100%）和高球蛋白血症（91%）。组织病理学显示以慢性肉芽肿性炎症浸润为主（63.6%），在30.3%的组织病理学检查和所进行的22次细胞学检查中的31.8%中检测到无鞭毛体形式。约72.7%的犬IIF血清学阳性，7只动物中有5只皮肤试验呈阳性。由于临床相似性、组织病理学和非特异性实验室检查结果相似性、患有孢子丝菌病的犬利什曼病血清学和皮肤试验呈阳性以及里约热内卢市流行区域重叠，这两种疾病的鉴别诊断需要证实其各自的病原体。
