Aquaculture Research Laboratory, Department of Zoology, Dr. Babasaheb Ambedkar Marathwada University, Maharashtra, India.
Mol Biol Rep. 2010 Mar;37(3):1355-62. doi: 10.1007/s11033-009-9517-3. Epub 2009 Apr 10.
Genetic similarity and diversity of catfish Clarias batrachus (Linn.1758) populations collected from three regions of Indian riverine system were examined using randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). Out of 22 random primers tested, six primers produced 462 RAPD bands ranging from 105 to 128 polymorphic bands per primer in size between 100 and 1,200 bp. The polymorphic bands in these populations ranged from 26.5 to 30.5. Polymorphic bands within populations ranged from 25 to 35.7%. The degree of similarity within Hussainabad population varied from 0.125 to 0.842 based on average level of band sharing (BS) values. The level of band sharing values within the catfish populations were 0.26 +/- 0.021 for Banaras, 0.60 +/- 0.033 for Bhubaneshwar and 0.377 +/- 0.058 for Hussainabad respectively. The results from the present study indicate that there is great degree of genetic similarity between Bhubaneshwar and Hussainabad populations where as Banaras catfish population is distinct. It may appear that Bhubaneshwar and Hussainabad are geographically connected by rivers and most of the major catfish hatcheries are located in this region, therefore the individuals from these populations are get reared in the same environmental conditions, migration or by inbreeding during several generations may be possible. This may be the reason that catfish population is lacking genetic diversity in major riverine system of India. In nearer future, the lack in genetic diversity can lead to inbreeding which can be resulted in poor growth and disease susceptibility, Bhubaneshwar and Hussainabad catfish population may have this problem.
从印度河流水系的三个地区采集的鲶鱼 Clarias batrachus（林奈 1758 年）种群的遗传相似性和多样性，使用随机扩增多态性 DNA-聚合酶链反应（RAPD-PCR）进行了检查。在测试的 22 个随机引物中，有 6 个引物产生了 462 个 RAPD 带，每个引物的大小在 100 到 1200bp 之间，有 105 到 128 个多态性带。这些种群中的多态性带范围为 26.5 到 30.5。种群内的多态性带范围为 25 到 35.7%。基于平均带共享（BS）值，Hussainabad 种群内的相似性程度从 0.125 到 0.842 不等。鲶鱼种群内的带共享值分别为 Banaras 为 0.26 +/- 0.021，Bhubaneshwar 为 0.60 +/- 0.033，Hussainabad 为 0.377 +/- 0.058。本研究结果表明，Bhubaneshwar 和 Hussainabad 种群之间存在很大程度的遗传相似性，而 Banaras 鲶鱼种群则不同。Bhubaneshwar 和 Hussainabad 可能通过河流在地理上相连，而且大多数主要的鲶鱼孵化场都位于该地区，因此，这些种群的个体在相同的环境条件下饲养，可能会发生迁移或几代近亲繁殖。这可能是印度主要河流水系中鲶鱼种群遗传多样性缺乏的原因。在不久的将来，遗传多样性的缺乏可能导致近交，这可能导致生长不良和易患病，Bhubaneshwar 和 Hussainabad 的鲶鱼种群可能存在这个问题。
