Wang Lih-Chiann, Chen Chi-Tsong, Lee Hsiao-Yuan, Li Shou-Hsien, Lir Jihn-Tsair, Chin Shin-Chien, Pu Chang-En, Wang Ching-Ho
Graduate Institute of Veterinary Medicine, Department of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.
Zoo Biol. 2007 Sep;26(5):425-31. doi: 10.1002/zoo.20149.
Identifying the sex of a bird is important to ensure successful breeding strategies and effective conservation programs. Sex may be identified from the intron size of the CHD1 gene located on the avian sex chromosomes Z and W. However, because of the great nucleotide diversity across different avian species, no given intron is in widespread use without ambiguous results. Complicated modifications of the reaction condition are required to suit different species. Two CHD1 introns were used with a unified reaction condition in this study to simplify the procedure. Consequently, genders of 73 avian species covering 19 families were successfully identified based on this two-intron approach. This means the ability to sex a wider range of avian species using a simplified procedure, greatly assisting in population management at zoos. Zoo Biol 26:425-431, 2007. (c) 2007 Wiley-Liss, Inc.
识别鸟类的性别对于确保成功的繁殖策略和有效的保护计划至关重要。可以通过位于鸟类性染色体Z和W上的CHD1基因的内含子大小来识别性别。然而，由于不同鸟类物种之间存在巨大的核苷酸多样性，没有一个特定的内含子能在不产生模糊结果的情况下被广泛使用。需要对反应条件进行复杂的调整以适应不同的物种。在本研究中，使用了两个CHD1内含子并采用统一的反应条件来简化程序。因此，基于这种双内含子方法成功识别了涵盖19个科的73种鸟类的性别。这意味着能够使用简化程序对更广泛的鸟类物种进行性别鉴定，极大地有助于动物园的种群管理。《动物园生物学》26:425 - 431，2007年。(c) 2007威利 - 利斯公司。
