糖尿病患者玻璃体液中血小板反应蛋白1和色素上皮衍生因子水平的调节
Modulation of thrombospondin 1 and pigment epithelium-derived factor levels in vitreous fluid of patients with diabetes.
作者信息
Wang Shoujian, Gottlieb Justin L, Sorenson Christine M, Sheibani Nader
机构信息
Department of Ophthalmology and Visual Sciences, University of Wisconsin School of Medicine and Public Health, 600 Highland Ave, K6/458 CSC, Madison, WI 53792-4673, USA.
出版信息
Arch Ophthalmol. 2009 Apr;127(4):507-13. doi: 10.1001/archophthalmol.2009.53.
OBJECTIVE
To determine the levels of 2 endogenous inhibitors of angiogenesis, thrombospondin 1 (TSP-1) and pigment epithelium-derived factor (PEDF), in the vitreous fluid from patients with and without diabetes.
METHODS
The levels of TSP-1 and PEDF in vitreous samples from diabetic and age-matched nondiabetic patients were determined by Western blot analysis.
RESULTS
We observed significant amounts of TSP-1 and PEDF in the vitreous samples of control eyes. The TSP-1 levels varied in samples from patients with diabetes. In contrast, PEDF levels showed little or no change in vitreous samples from patients with or without diabetes. However, the PEDF protein exhibited variation in its molecular weight among the samples. We consistently observed lower levels of TSP-1 in diabetic patients who expressed the higher-molecular-weight PEDF isoform.
CONCLUSIONS
In diabetes, changes in the TSP-1 level may play a role in shifting the angiogenic balance and contributing to the pathogenesis of diabetic retinopathy. Although the PEDF level did not change, the diabetic samples with the higher-molecular-weight PEDF isoform consistently showed lower levels of TSP-1.
CLINICAL RELEVANCE
The presence of the higher-molecular-weight PEDF isoform may be associated with greater risk of severe diabetic retinopathy.
目的
测定糖尿病患者和非糖尿病患者玻璃体液中两种内源性血管生成抑制剂——血小板反应蛋白1（TSP-1）和色素上皮衍生因子（PEDF）的水平。
方法
采用蛋白质印迹分析测定糖尿病患者和年龄匹配的非糖尿病患者玻璃体液样本中TSP-1和PEDF的水平。
结果
我们在对照眼的玻璃体液样本中观察到大量的TSP-1和PEDF。糖尿病患者样本中的TSP-1水平各不相同。相比之下，无论糖尿病患者与否，其玻璃体液样本中的PEDF水平几乎没有变化或没有变化。然而，PEDF蛋白在各样本中的分子量存在差异。我们始终观察到，表达高分子量PEDF异构体的糖尿病患者体内TSP-1水平较低。
结论
在糖尿病中，TSP-1水平的变化可能在改变血管生成平衡及促成糖尿病视网膜病变的发病机制中发挥作用。虽然PEDF水平没有变化，但具有高分子量PEDF异构体的糖尿病样本始终显示出较低的TSP-1水平。
临床意义
高分子量PEDF异构体的存在可能与严重糖尿病视网膜病变的更大风险相关。
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