Swiston Albert J, Cheng Connie, Um Soong Ho, Irvine Darrell J, Cohen Robert E, Rubner Michael F
Department of Materials Science and Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Nano Lett. 2008 Dec;8(12):4446-53. doi: 10.1021/nl802404h.
We demonstrate that functional polyelectrolyte multilayer (PEM) patches can be attached to a fraction of the surface area of living, individual lymphocytes. Surface-modified cells remain viable at least 48 h following attachment of the functional patch, and patches carrying magnetic nanoparticles allow the cells to be spatially manipulated using a magnetic field. The patch does not completely occlude the cellular surface from the surrounding environment; this approach allows a functional payload to be attached to a cell that is still free to perform its native functions, as suggested by preliminary studies on patch-modified T-cell migration. This approach has potential for broad applications in bioimaging, cellular functionalization, immune system and tissue engineering, and cell-based therapeutics where cell-environment interactions are critical.
我们证明，功能性聚电解质多层（PEM）贴片可以附着在单个活淋巴细胞的部分表面积上。在附着功能性贴片后，表面修饰的细胞至少在48小时内保持存活，并且携带磁性纳米颗粒的贴片允许使用磁场对细胞进行空间操纵。贴片不会完全将细胞表面与周围环境隔离开；正如对贴片修饰的T细胞迁移的初步研究所表明的，这种方法允许将功能性负载附着到仍然能够自由执行其天然功能的细胞上。这种方法在生物成像、细胞功能化、免疫系统和组织工程以及细胞与环境相互作用至关重要的基于细胞的治疗等广泛应用中具有潜力。
