Kalkowski I, Conrad R
Universität Konstanz, Fakultät für Biologie, F.R.G.
FEMS Microbiol Lett. 1991 Jul 15;66(1):107-11. doi: 10.1016/0378-1097(91)90429-e.
Suspensions of Pseudomonas aeruginosa and Bacillus cereus were continuously sparged with nitrogen to remove gaseous products of nitrate reduction. Under these conditions, P. aeruginosa denitrified nitrate to 4% NO, 47% N2O and 49% N2. B. cereus reduced nitrate to 94% nitrite, 2% NO and 5% N2O. B. cereus was unable to reduce NO or N2O as sole electron acceptor, whereas P. aeruginosa reduced NO stoichiometrically to N2O when the N2O reductase was inhibited by acetylene or when the formed N2O was immediately flushed out of the incubation vessel. The mechanism and the reason for NO production in nitrate-respiring B. cereus are unknown, but the amounts of NO released were in a similar range as in the denitrifying P. aeruginosa and thus may be of similar environmental importance.
用氮气持续鼓泡铜绿假单胞菌和蜡样芽孢杆菌的悬浮液，以去除硝酸盐还原的气态产物。在这些条件下，铜绿假单胞菌将硝酸盐反硝化生成4%的一氧化氮（NO）、47%的一氧化二氮（N₂O）和49%的氮气（N₂）。蜡样芽孢杆菌将硝酸盐还原为94%的亚硝酸盐、2%的NO和5%的N₂O。蜡样芽孢杆菌不能将NO或N₂O作为唯一电子受体进行还原，而当N₂O还原酶被乙炔抑制或形成的N₂O立即从培养容器中冲洗掉时，铜绿假单胞菌能将NO化学计量地还原为N₂O。在进行硝酸盐呼吸的蜡样芽孢杆菌中产生NO的机制和原因尚不清楚，但释放的NO量与反硝化的铜绿假单胞菌处于相似范围内，因此可能具有相似的环境重要性。
