反硝化铜绿假单胞菌中硝酸盐吸收的氧调节
Oxygen regulation of nitrate uptake in denitrifying Pseudomonas aeruginosa.
作者信息
Hernandez D, Rowe J J
出版信息
Appl Environ Microbiol. 1987 Apr;53(4):745-50. doi: 10.1128/aem.53.4.745-750.1987.
Abstract
Oxygen had an immediate and reversible inhibitory effect on nitrate respiration by denitrifying cultures of Pseudomonas aeruginosa. Inhibition of nitrate utilization by oxygen appeared to be at the level of nitrate uptake, since nitrate reduction to nitrite in cell extracts was not affected by oxygen. The degree of oxygen inhibition was dependent on the concentration of oxygen, and increasing nitrate concentrations could not overcome the inhibition. The inhibitory effect of oxygen was maximal at approximately 0.2% oxygen saturation. The inhibition appeared to be specific for nitrate uptake. Nitrite uptake was not affected by these low levels of aeration, and nitrite reduction was only partially inhibited in the presence of oxygen. The regulation of nitrate respiration at the level of transport by oxygen may represent a major mechanism by which the entire denitrification pathway is regulated in P. aeruginosa.
摘要
氧气对铜绿假单胞菌反硝化培养物的硝酸盐呼吸具有即时且可逆的抑制作用。氧气对硝酸盐利用的抑制似乎发生在硝酸盐摄取水平，因为细胞提取物中硝酸盐还原为亚硝酸盐不受氧气影响。氧气抑制的程度取决于氧气浓度，增加硝酸盐浓度无法克服这种抑制。氧气的抑制作用在约0.2%的氧饱和度时最大。这种抑制似乎对硝酸盐摄取具有特异性。亚硝酸盐摄取不受这些低水平通气的影响，并且在有氧气存在时亚硝酸盐还原仅受到部分抑制。氧气在转运水平对硝酸盐呼吸的调节可能是铜绿假单胞菌中整个反硝化途径被调节的主要机制。
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