Nishikawa Tasuku, Doi Kiyoshi, Ochi Naoki, Katsunuma Sayaka, Nibu Ken-ichi
Department of Otolaryngology-Head and Neck Surgery, Kobe University Graduate School of Medicine, Kobe, Japan.
Neuroreport. 2009 May 27;20(8):764-9. doi: 10.1097/WNR.0b013e32832b169e.
To further study the effects of basic fibroblast growth factor (bFGF) on the olfactory epithelium, bFGF was intranasally administered twice a day for 6 weeks to 2.5-month-old and 7-month-old mice. The effects were immunohistochemically examined by using antibodies against proliferating cell nuclear antigen, olfactory marker protein, and GAP43. The number of cells positive for proliferating cell nuclear antigen in the supporting cell layer increased dramatically, and that of GAP43-positive cells, or globose basal cells, increased significantly, especially in aging mice. However, no significant changes were observed in the number of olfactory marker protein-positive cells or mature olfactory receptor neurons. These results suggest that topical application of bFGF promotes proliferation of globose basal cells and supporting cells.
为进一步研究碱性成纤维细胞生长因子（bFGF）对嗅上皮的影响，对2.5月龄和7月龄小鼠每天经鼻给予bFGF两次，持续6周。通过使用抗增殖细胞核抗原、嗅觉标记蛋白和GAP43的抗体进行免疫组织化学检查来观察其效果。支持细胞层中增殖细胞核抗原阳性细胞的数量显著增加，GAP43阳性细胞（即球状基底细胞）的数量也显著增加，尤其是在老龄小鼠中。然而，嗅觉标记蛋白阳性细胞或成熟嗅觉受体神经元的数量未观察到显著变化。这些结果表明，局部应用bFGF可促进球状基底细胞和支持细胞的增殖。
