Hirayama T, Imajuku Y, Anai T, Matsui M, Oka A
Institute for Chemical Research, Kyoto University, Japan.
Gene. 1991 Sep 15;105(2):159-65. doi: 10.1016/0378-1119(91)90146-3.
The cdc2 gene product (p34cdc2) has been thought to play a central role in control of the mitotic cell cycle of yeasts and animals. To approach an understanding of the cell-cycle-control system in higher plants, we isolated, from an Arabidopsis thaliana cDNA library, two clones (CDC2a and CDC2b) similar to the Schizosaccharomyces pombe cdc2 gene. Genomic Southern-blot analysis with the CDC2a and CDC2b cDNA probes suggested that the A. thaliana genome contains several additional cdc2-like genes, which together with the CDC2a and CDC2b genes may constitute a CDC2 gene family. The CDC2a cDNA expressed in Sc. pombe corrected the elongated morphology, caused by the temperature-sensitive cdc2-33 mutation, to the normal shapes, indicating that the A. thaliana CDC2a gene product resembles Sc. pombe p34cdc2 functionally as well as structurally. These results support the view that the cell cycle of higher plants is controlled by an analogue of a p34cdc2-centered regulatory system like that of yeasts and animals.
人们一直认为细胞周期蛋白依赖性激酶2基因产物（p34cdc2）在酵母和动物有丝分裂细胞周期的控制中起核心作用。为了深入了解高等植物的细胞周期控制系统，我们从拟南芥cDNA文库中分离出两个与粟酒裂殖酵母cdc2基因相似的克隆（CDC2a和CDC2b）。用CDC2a和CDC2b cDNA探针进行的基因组Southern杂交分析表明，拟南芥基因组含有几个额外的cdc2样基因，这些基因与CDC2a和CDC2b基因一起可能构成一个CDC2基因家族。在粟酒裂殖酵母中表达的CDC2a cDNA将由温度敏感型cdc2-33突变引起的细长形态恢复为正常形状，这表明拟南芥CDC2a基因产物在功能和结构上与粟酒裂殖酵母p34cdc2相似。这些结果支持这样一种观点，即高等植物的细胞周期受类似于酵母和动物中以p34cdc2为中心的调节系统的调控。
