Ferreira P C, Hemerly A S, Villarroel R, Van Montagu M, Inzé D
Laboratorium voor Genetica, Rijksuniversiteit Gent, Belgium.
Plant Cell. 1991 May;3(5):531-40. doi: 10.1105/tpc.3.5.531.
The p34cdc2 protein kinase is a key component of the eukaryotic cell cycle, which is required for G1 to S-phase transition and for entry into mitosis. Using a 380-base pair DNA fragment obtained by polymerase chain reaction amplification from an Arabidopsis thaliana flower cDNA library as a probe, we isolated and sequenced a cdc2-homologous cDNA from Arabidopsis. The encoded polypeptide has extensive homology with cdc2-like kinases. Furthermore, when expressed in a CDC28ts Saccharomyces strain, it partially restores the capacity to grow at 36 degrees C, indicating that the plant cDNA is a functional homolog of the p34cdc2 kinase. Genomic hybridization demonstrated that there is one copy of the cdc2 gene per Arabidopsis haploid genome. Using RNA gel blot analysis, we found that cdc2 mRNA is present in all plant organs.
p34cdc2蛋白激酶是真核细胞周期的关键组成部分，G1期到S期的转换以及进入有丝分裂都需要它。我们以通过聚合酶链反应扩增从拟南芥花cDNA文库获得的一段380个碱基对的DNA片段为探针，从拟南芥中分离并测序了一个与cdc2同源的cDNA。编码的多肽与cdc2样激酶具有广泛的同源性。此外，当在CDC28ts酿酒酵母菌株中表达时，它部分恢复了在36℃下生长的能力，表明该植物cDNA是p34cdc2激酶的功能同源物。基因组杂交表明，每个拟南芥单倍体基因组中有一个cdc2基因拷贝。通过RNA凝胶印迹分析，我们发现cdc2 mRNA存在于所有植物器官中。
