Kishore R Krishna, Sudhakar D, Parthasarathy P R
Department of Biochemistry, Sri Venkateswara University, Tirupati.517502, A.P, India.
Indian J Biochem Biophys. 2009 Feb;46(1):106-11.
The possible protective role of pomegranate (Punica granatum L.) fruit extract which has shown antioxidant capacity higher than that of red wine and green tea was evaluated against adriamycin-induced oxidative stress in chick embryos. Adriamycin (ADR), an anthracycline broad spectrum of chemotherapeutic drug is used for the treatment of variety of cancers; however, its prolonged use is limited by an irreversible, dose-dependant and progressive cardiomyopathy, hepatotoxicity and general toxicity to other organs in human beings, due to oxidative stress. The morphological changes (malformation of different organs), changes in body weight, volume of amniotic fluid (AF) and biochemical parameters of AF were studied after 24 and 48 h of incubation by comparing ADR alone and pomegranate fruit extract pretreated groups with their respective controls of 12 days old chick embryos. ADR alone at a dose of 70 microg/egg showed a significant dose versus time- dependent reduction in body weight, volume of AF. A dose-related increase in embryo gross morphological deformities and significant changes in the levels of biochemical parameters in AF were observed in ADR-treated group. These changes were significantly ameliorated to normal by pre-administration of pomegranate fruit extract at a dose of 200 microg/egg. Thus, the present study demonstrated the embryo protective nature of pomegranate fruit extract against ADR-induced oxidative stress.
石榴（Punica granatum L.）果实提取物已显示出高于红酒和绿茶的抗氧化能力，本研究评估了其对阿霉素诱导的鸡胚氧化应激的潜在保护作用。阿霉素（ADR）是一种蒽环类广谱化疗药物，用于治疗多种癌症；然而，由于氧化应激，其长期使用受到不可逆的、剂量依赖性和进行性心肌病、肝毒性以及对人体其他器官的一般毒性的限制。通过比较单独使用阿霉素组和石榴果实提取物预处理组与其各自12日龄鸡胚对照组，在孵化24小时和48小时后研究了形态学变化（不同器官的畸形）、体重变化、羊水（AF）体积和AF的生化参数。单独使用70微克/蛋剂量的阿霉素显示出体重和AF体积随剂量和时间的显著降低。在阿霉素处理组中观察到胚胎总体形态畸形的剂量相关增加以及AF中生化参数水平的显著变化。通过预先给予200微克/蛋剂量的石榴果实提取物，这些变化显著改善至正常。因此，本研究证明了石榴果实提取物对阿霉素诱导的氧化应激具有胚胎保护作用。
