Piasecki Shawn K, Hall Bradley, Ellington Andrew D
Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX, USA.
Methods Mol Biol. 2009;535:3-18. doi: 10.1007/978-1-59745-557-2_1.
Random sequence nucleic acid pools can be used in a variety of applications, including the selection of functional nucleic acids such as protein binding sites, aptamers, and ribozymes. while the design, synthesis, and purification of pools is relatively straightforward, keeping track of the size and complexity of a nucleic acid pool can sometimes task even an experienced researcher. The following protocol takes the reader through the steps necessary for the preparation of a pool of known complexity.
随机序列核酸文库可用于多种应用，包括选择功能性核酸，如蛋白质结合位点、适体和核酶。虽然文库的设计、合成和纯化相对简单，但即使是经验丰富的研究人员，有时也难以追踪核酸文库的大小和复杂性。以下方案将引导读者完成制备已知复杂性文库所需的步骤。
