Margetis Panagiotis I, Antonelou Marianna H, Petropoulos Ioannis K, Margaritis Lukas H, Papassideri Issidora S
Department of Cell Biology and Biophysics, Faculty of Biology, University of Athens, Athens, Greece.
Exp Mol Pathol. 2009 Aug;87(1):76-82. doi: 10.1016/j.yexmp.2009.04.001. Epub 2009 Apr 18.
We investigated the protein carbonylation of red blood cell (RBC) membrane in type 2 diabetic patients and the potential implication of carbonyl/oxidative stress in reflecting disease severity. Sixty-four diabetic patients with or without retinopathy of variable clinical severity (Groups DR and DM, respectively) and 20 healthy controls were included in the study. Protein carbonyls were determined in RBC membranes by immunoblotting. Compared to healthy volunteers, the RBC membranes of diabetic patients were characterized by significantly increased levels of carbonylated proteins. The carbonylation of Group DR was higher compared to that of Group DM. The subgroup of patients with proliferative retinopathy exhibited a trend towards a significant increase in protein carbonyls, compared to both free-of-retinopathy diabetic cases and non-proliferative diabetic retinopathy cases. The correlation between the chemical modifications of the erythrocyte membrane proteins and the clinical severity of diabetic retinopathy suggests a potential utility of membrane carbonylation as a marker and risk factor in the development of retinopathy.
我们研究了2型糖尿病患者红细胞（RBC）膜的蛋白质羰基化以及羰基/氧化应激在反映疾病严重程度方面的潜在意义。本研究纳入了64例患有不同临床严重程度视网膜病变的糖尿病患者（分别为DR组和DM组）以及20名健康对照者。通过免疫印迹法测定红细胞膜中的蛋白质羰基。与健康志愿者相比，糖尿病患者的红细胞膜具有羰基化蛋白水平显著升高的特征。DR组的羰基化程度高于DM组。与无视网膜病变的糖尿病病例和非增殖性糖尿病视网膜病变病例相比，增殖性视网膜病变患者亚组的蛋白质羰基有显著增加的趋势。红细胞膜蛋白的化学修饰与糖尿病视网膜病变临床严重程度之间的相关性表明，膜羰基化作为视网膜病变发生发展的标志物和危险因素具有潜在用途。
