枯草芽孢杆菌染色体上dnaB基因及相邻基因的核苷酸序列与组织。
Nucleotide sequence and organization of dnaB gene and neighbouring genes on the Bacillus subtilis chromosome.
作者信息
Ogasawara N, Moriya S, Mazza P G, Yoshikawa H
出版信息
Nucleic Acids Res. 1986 Dec 22;14(24):9989-99. doi: 10.1093/nar/14.24.9989.
Abstract
A region of the Bacillus subtilis chromosome containing dnaB, a gene essential for the initiation of chromosomal replication in B. subtilis, was cloned and nucleotide sequence of some 5000bp determined. The region consists of 4 open reading frames (ORFs) possibly comprising a single transcriptional unit. Two dnaB mutations, dnaB27 and dnaB19 were located within the first ORF which would give rise to a protein of 472 amino acids. The dnaB27 mutation involves codon at the position of 122 (replacement of Asp by Asn) close to a DNA binding domain and the dnaB19 codon 379 (replacement of Ala by Thr) close to a region rich in charged amino acids which may be alpha helical. The third ORF in the same transcriptional unit would produce a hydrophobic protein which might be involved in the DNA-membrane binding function of dnaB gene. No homologous Escherichia coli genes have been found.
摘要
枯草芽孢杆菌染色体中包含dnaB的区域被克隆，该区域对枯草芽孢杆菌染色体复制的起始至关重要，并且测定了约5000bp的核苷酸序列。该区域由4个开放阅读框（ORF）组成，可能构成一个单一的转录单元。两个dnaB突变体，dnaB27和dnaB19，位于第一个ORF内，该ORF会产生一个由472个氨基酸组成的蛋白质。dnaB27突变涉及第122位密码子（天冬氨酸被天冬酰胺取代），靠近一个DNA结合结构域；而dnaB19突变涉及第379位密码子（丙氨酸被苏氨酸取代），靠近一个富含带电荷氨基酸的区域，该区域可能是α螺旋结构。同一转录单元中的第三个ORF会产生一种疏水蛋白，它可能参与dnaB基因的DNA-膜结合功能。未发现与大肠杆菌同源的基因。
相似文献
1
Nucleotide sequence and organization of dnaB gene and neighbouring genes on the Bacillus subtilis chromosome.枯草芽孢杆菌染色体上dnaB基因及相邻基因的核苷酸序列与组织。
Nucleic Acids Res. 1986 Dec 22;14(24):9989-99. doi: 10.1093/nar/14.24.9989.
2
Nucleotide sequence of Bacillus subtilis dnaB: a gene essential for DNA replication initiation and membrane attachment.枯草芽孢杆菌dnaB的核苷酸序列：DNA复制起始和膜附着所必需的基因。
Proc Natl Acad Sci U S A. 1987 Feb;84(3):653-7. doi: 10.1073/pnas.84.3.653.
3
The Bacillus subtilis dnaC gene encodes a protein homologous to the DnaB helicase of Escherichia coli.枯草芽孢杆菌的dnaC基因编码一种与大肠杆菌DnaB解旋酶同源的蛋白质。
Microbiology (Reading). 1995 Mar;141 ( Pt 3):641-4. doi: 10.1099/13500872-141-3-641.
4
Genes and their organization in the replication origin region of the bacterial chromosome.细菌染色体复制起始区域中的基因及其组织形式。
Mol Microbiol. 1992 Mar;6(5):629-34. doi: 10.1111/j.1365-2958.1992.tb01510.x.
5
Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin.对枯草芽孢杆菌染色体中包含复制起点的180千碱基区域进行系统测序。
DNA Res. 1994;1(1):1-14. doi: 10.1093/dnares/1.1.1.
6
Conservation of genes and their organization in the chromosomal replication origin region of Bacillus subtilis and Escherichia coli.枯草芽孢杆菌和大肠杆菌染色体复制起始区域中基因及其组织的保守性。
EMBO J. 1985 Dec 1;4(12):3345-50. doi: 10.1002/j.1460-2075.1985.tb04087.x.
7
In vitro type II binding of chromosomal DNA to membrane in Bacillus subtilis.枯草芽孢杆菌中染色体DNA与细胞膜的体外II型结合
J Bacteriol. 1991 Dec;173(23):7732-5. doi: 10.1128/jb.173.23.7732-7735.1991.
8
Cloning and characterization of a Bacillus subtilis gene encoding a homolog of the 54-kilodalton subunit of mammalian signal recognition particle and Escherichia coli Ffh.枯草芽孢杆菌中一个编码哺乳动物信号识别颗粒54千道尔顿亚基及大肠杆菌Ffh同源物的基因的克隆与特性分析
J Bacteriol. 1993 Aug;175(15):4885-94. doi: 10.1128/jb.175.15.4885-4894.1993.
9
Cloning and characterization of a Bacillus subtilis gene homologous to E. coli secY.枯草芽孢杆菌中与大肠杆菌secY同源的一个基因的克隆与特性分析
J Biochem. 1990 Apr;107(4):603-7. doi: 10.1093/oxfordjournals.jbchem.a123093.
10
A Bacillus subtilis spore coat polypeptide gene, cotS.一个枯草芽孢杆菌芽孢衣多肽基因，cotS。
Microbiology (Reading). 1995 Jun;141 ( Pt 6):1433-1442. doi: 10.1099/13500872-141-6-1433.
引用本文的文献
1
The metabolic enzyme ManA reveals a link between cell wall integrity and chromosome morphology.代谢酶 ManA 揭示了细胞壁完整性和染色体形态之间的联系。
PLoS Genet. 2010 Sep 16;6(9):e1001119. doi: 10.1371/journal.pgen.1001119.
2
DnaB proteolysis in vivo regulates oligomerization and its localization at oriC in Bacillus subtilis.体内 DnaB 蛋白水解调节寡聚化及其在枯草芽孢杆菌 oriC 处的定位。
Nucleic Acids Res. 2010 May;38(9):2851-64. doi: 10.1093/nar/gkp1236. Epub 2010 Jan 13.
3
Identification of temperature-sensitive dnaD mutants of Staphylococcus aureus that are defective in chromosomal DNA replication.金黄色葡萄球菌温度敏感型dnaD突变体的鉴定，这些突变体在染色体DNA复制方面存在缺陷。
Mol Genet Genomics. 2004 May;271(4):447-57. doi: 10.1007/s00438-004-0996-6. Epub 2004 Mar 24.
4
DnaD protein of Bacillus subtilis interacts with DnaA, the initiator protein of replication.枯草芽孢杆菌的DnaD蛋白与复制起始蛋白DnaA相互作用。
J Bacteriol. 2001 Mar;183(6):2148-50. doi: 10.1128/JB.183.6.2148-2150.2001.
5
Suppression of initiation defects of chromosome replication in Bacillus subtilis dnaA and oriC-deleted mutants by integration of a plasmid replicon into the chromosomes.通过将质粒复制子整合到染色体中抑制枯草芽孢杆菌dnaA和oriC缺失突变体中染色体复制起始缺陷。
J Bacteriol. 1997 Apr;179(8):2494-502. doi: 10.1128/jb.179.8.2494-2502.1997.
6
Expression of both Bacillus subtilis threonyl-tRNA synthetase genes is autogenously regulated.枯草芽孢杆菌苏氨酰 - tRNA合成酶两个基因的表达是自身调控的。
J Bacteriol. 1994 Jan;176(2):486-94. doi: 10.1128/jb.176.2.486-494.1994.
7
Flanking sequences affect replication arrest at the Escherichia coli terminator TerB in vivo.侧翼序列在体内影响大肠杆菌终止子TerB处的复制停滞。
J Bacteriol. 1994 Jul;176(13):4165-7. doi: 10.1128/jb.176.13.4165-4167.1994.
8
Primosome assembly site in Bacillus subtilis.枯草芽孢杆菌中的引发体装配位点。
EMBO J. 1995 Jun 1;14(11):2642-50. doi: 10.1002/j.1460-2075.1995.tb07262.x.
9
Compilation and analysis of Bacillus subtilis sigma A-dependent promoter sequences: evidence for extended contact between RNA polymerase and upstream promoter DNA.枯草芽孢杆菌σA依赖型启动子序列的汇编与分析：RNA聚合酶与上游启动子DNA之间存在广泛接触的证据
Nucleic Acids Res. 1995 Jul 11;23(13):2351-60. doi: 10.1093/nar/23.13.2351.
10
Molecular cloning of Bacillus subtilis genes involved in DNA metabolism.参与DNA代谢的枯草芽孢杆菌基因的分子克隆
Mol Gen Genet. 1987 Aug;209(1):8-14. doi: 10.1007/BF00329829.
本文引用的文献
1
Genetic mapping of a group of temperature-sensitive dna initiation mutants in Bacillus subtilis.枯草芽孢杆菌中一组温度敏感型DNA起始突变体的遗传图谱分析。
Genetics. 1980 Apr;94(4):809-23. doi: 10.1093/genetics/94.4.809.
2
DNA-membrane association is necessary for initiation of chromosomal and plasmid replication in Bacillus subtilis.DNA与膜的结合对于枯草芽孢杆菌中染色体和质粒复制的起始是必需的。
Proc Natl Acad Sci U S A. 1980 May;77(5):2834-8. doi: 10.1073/pnas.77.5.2834.
3
Structural and transcriptional evidence for related thrS and infC expression.thrS和infC相关表达的结构和转录证据。
Proc Natl Acad Sci U S A. 1983 Oct;80(20):6152-6. doi: 10.1073/pnas.80.20.6152.
4
Construction and properties of an integrable plasmid for Bacillus subtilis.枯草芽孢杆菌可整合质粒的构建与特性
J Bacteriol. 1983 Jun;154(3):1513-5. doi: 10.1128/jb.154.3.1513-1515.1983.
5
Protein-DNA recognition.蛋白质-脱氧核糖核酸识别
Annu Rev Biochem. 1984;53:293-321. doi: 10.1146/annurev.bi.53.070184.001453.
6
Isolation and genetic analysis of temperature-sensitive mutants of B. subtilis defective in DNA synthesis.枯草芽孢杆菌DNA合成缺陷型温度敏感突变体的分离与遗传分析。
Mol Gen Genet. 1970;108(3):277-87. doi: 10.1007/BF00283358.
7
Characterization of a temperature-sensitive mutant of Bacillus subtilis defective in deoxyribonucleic acid replication.枯草芽孢杆菌中一种在脱氧核糖核酸复制方面存在缺陷的温度敏感突变体的特性分析。
J Bacteriol. 1967 Nov;94(5):1603-8. doi: 10.1128/jb.94.5.1603-1608.1967.
8
The initiation of DNA replication in Bacillus subtilis.枯草芽孢杆菌中DNA复制的起始
Proc Natl Acad Sci U S A. 1967 Jul;58(1):312-9. doi: 10.1073/pnas.58.1.312.
9
Temperature-sensitive DNA synthesis mutants of Bacillus subtilis--appendix: theory of density transfer for symmetric chromosome replication.枯草芽孢杆菌的温度敏感型DNA合成突变体——附录：对称染色体复制的密度转移理论
Genetics. 1973 Feb;73(2):185-214. doi: 10.1093/genetics/73.2.185.
10
Temperature-sensitive initiation of chromosome replication in a mutant of Bacillus subtilis.枯草芽孢杆菌突变体中染色体复制的温度敏感起始
J Bacteriol. 1973 May;114(2):474-84. doi: 10.1128/jb.114.2.474-484.1973.
Zotero 插件