Gu L Q, Cheley S, Bayley H
Department of Medical Biochemistry and Genetics, Texas A&M University System Health Science Center, College Station, TX 77843, USA.
Science. 2001 Jan 26;291(5504):636-40. doi: 10.1126/science.291.5504.636.
We describe a heptameric protein pore that has been engineered to accommodate two different cyclodextrin adapters simultaneously within the lumen of a transmembrane beta barrel. The volume between the adapters is a cavity of approximately 4400 cubic angstroms. Analysis of single-channel recordings reveals that individual charged organic molecules can be pulled into the cavity by an electrical potential. Once trapped, an organic molecule shuttles back and forth between the adapters for hundreds of milliseconds. Such self-assembling nanostructures are of interest for the fabrication of multianalyte sensors and could provide a means to control chemical reactions.
我们描述了一种七聚体蛋白质孔道，它经过工程设计，能够在跨膜β桶的腔内同时容纳两种不同的环糊精适配体。适配体之间的空间是一个体积约为4400立方埃的腔。单通道记录分析表明，单个带电有机分子可通过电势被拉入腔内。一旦被捕获，有机分子会在适配体之间来回穿梭数百毫秒。这种自组装纳米结构对于制造多分析物传感器很有意义，并且可能提供一种控制化学反应的方法。
