Rahimi Pooneh, Tabatabaie H, Gouya Mohammad M, Mahmudi M, Musavi T, Rad K Samimi, Azad T Mokhtari, Nategh R
Department of Virology, Pasteur Institute of Iran, Tehran, Iran.
J Clin Virol. 2009 Jun;45(2):139-41. doi: 10.1016/j.jcv.2009.03.004. Epub 2009 Apr 24.
The 66 serotypes of human enteroviruses (EVs) are classified into four species A-D, based on phylogenetic relationships in multiple genome regions. Partial VP(1) amplification and sequence analysis are reliable methods for identifying non-polio enterovirus serotypes, especially in negative cell culture specimens from patients with residual paralysis.
In Iran during the years 2000-2002, there were 29 residual paralysis cases with negative cell (RD, HEp(2) and L(20)B) culture results.
The genomic RNA was extracted from stool specimens from cases of residual paralysis and detected by amplification of the 5'-nontranslated region using RT-PCR with Pan-EV primers. Partial VP(1) amplification by semi-nested RT-PCR (snRT-PCR) and sequence analysis were done.
Specimens from the 29 culture-negative cases contained echoviruses of six different serotypes.
The global eradication of wild polioviruses is near and study of non-polio enteroviruses, which can cause poliomyelitis, is increasingly important to understand their pathogenesis. The VP(1) sequences, derived from the snRT-PCR products, allowed rapid molecular analysis of these non-polio strains.
人类肠道病毒（EV）的66个血清型根据多个基因组区域的系统发育关系分为A - D四个种。部分VP(1)扩增和序列分析是鉴定非脊髓灰质炎肠道病毒血清型的可靠方法，尤其是在来自残留麻痹患者的阴性细胞培养标本中。
在2000 - 2002年期间的伊朗，有29例残留麻痹病例，其细胞（RD、HEp(2)和L(20)B）培养结果为阴性。
从残留麻痹病例的粪便标本中提取基因组RNA，使用泛肠道病毒引物通过RT - PCR扩增5' - 非翻译区进行检测。通过半巢式RT - PCR（snRT - PCR）进行部分VP(1)扩增并进行序列分析。
29例培养阴性病例的标本中含有六种不同血清型的埃可病毒。
全球根除野生脊髓灰质炎病毒已近在咫尺，而对可导致脊髓灰质炎的非脊髓灰质炎肠道病毒的研究对于了解其发病机制变得越来越重要。从snRT - PCR产物获得的VP(1)序列能够对这些非脊髓灰质炎毒株进行快速分子分析。
