de Jong G J
J Chromatogr. 1980 Aug 8;183(2):203-11. doi: 10.1016/s0378-4347(00)81694-1.
A high-performance liquid chromatographic system for the direct determination of the antidepressants clovoxamine and fluvoxamine in plasma is described. The primary amines are derivatized with the fluorogenic reagent fluorescamine in order to increase the sensitivity and selectivity, but also to decrease the polarity of the amines. The band broadening of some combinations of pre-column and analytical column is compared for the fluorescamine derivative of fluvoxamine. The combination of a pre-column containing RP-2 and an analytical column containing RP-8 has been successfully used for the determination of the two antidepressants in plasma. At relatively low concentrations of the drugs, a simple step-gradient elution is required for the removal of a large proportion of the more polar components of the samples. Concentrations in the range of 10-1000 ng clovoxamine per ml plasma were determined by means of external standardization and show a good correlation with the data of a more laborious gas chromatographic method. The detection limits for clovoxamine and fluvoxamine are approximately 3 ng/ml in plasma.
本文描述了一种用于直接测定血浆中抗抑郁药氯伏胺和氟伏沙明的高效液相色谱系统。伯胺用荧光试剂荧光胺进行衍生化，以提高灵敏度和选择性，同时降低胺的极性。比较了氟伏沙明荧光胺衍生物的一些预柱和分析柱组合的谱带展宽情况。含有RP - 2的预柱和含有RP - 8的分析柱的组合已成功用于测定血浆中的两种抗抑郁药。在相对较低的药物浓度下，需要简单的梯度洗脱来去除大部分极性较强的样品成分。通过外标法测定血浆中氯伏胺的浓度范围为每毫升血浆10 - 1000纳克，与更繁琐的气相色谱法数据显示出良好的相关性。氯伏胺和氟伏沙明在血浆中的检测限约为3纳克/毫升。
