Wiederkehr F
Institute of Clinical Chemistry, University Hospital Zürich, Switzerland.
J Chromatogr. 1991 Sep 13;569(1-2):281-96. doi: 10.1016/0378-4347(91)80234-4.
The cerebrospinal fluid (CSF) is a specific ultrafiltrate of plasma, which surrounds the brain and spinal cord. The study of its proteins and their alteration may yield useful information on several neurological diseases. By using various electrophoretic separation techniques, several CSF proteins have been identified derived from plasma or from brain. Different one-dimensional methods, such as agarose gel electrophoresis and isoelectric focusing, are of similar value in identifying the non-specific oligoclonal bands, which are mainly helpful in the diagnosis of multiple sclerosis and other inflammatory diseases. Isoelectric focusing has a greater resolution than other one-dimensional methods, and it yields additional data about disease-associated proteins occurring in Alzheimer's disease, Huntington's chorea and amyotrophic lateral sclerosis. Silver-stained two-dimensional gels provide more information about the complex protein composition of CSF, particularly about proteins produced in the brain, such as apolipoprotein E and neuron-specific enolase. For the detection of oligoclonal antibodies, the investigation of protein changes revealed by Parkinson's disease, schizophrenia and Creutzfeldt-Jakob disease, and the analysis of CSF immune complexes, two-dimensional electrophoresis has a greater sensitivity.
脑脊液（CSF）是血浆的一种特殊超滤液，它环绕着脑和脊髓。对其蛋白质及其变化的研究可能会产生有关多种神经系统疾病的有用信息。通过使用各种电泳分离技术，已鉴定出几种源自血浆或脑的脑脊液蛋白质。不同的一维方法，如琼脂糖凝胶电泳和等电聚焦，在鉴定非特异性寡克隆带方面具有相似的价值，这些带主要有助于多发性硬化症和其他炎症性疾病的诊断。等电聚焦比其他一维方法具有更高的分辨率，并且它能提供有关阿尔茨海默病、亨廷顿舞蹈病和肌萎缩侧索硬化症中出现的疾病相关蛋白质的额外数据。银染二维凝胶能提供更多有关脑脊液复杂蛋白质组成的信息，特别是有关脑中产生的蛋白质，如载脂蛋白E和神经元特异性烯醇化酶。对于检测寡克隆抗体、研究帕金森病、精神分裂症和克雅氏病所揭示的蛋白质变化以及分析脑脊液免疫复合物，二维电泳具有更高的灵敏度。
