痴呆患者血浆和脑脊液中的等电聚焦和二维凝胶电泳

Isoelectric focusing and two-dimensional gel electrophoresis in plasma and cerebrospinal fluid from patients with dementia.

作者信息

Alafuzoff I, Adolfsson R, Bucht G, Jellum E, Mehta P D, Winblad B

出版信息

Eur Neurol. 1986;25(4):285-9. doi: 10.1159/000116023.

Abstract

IgG profiles of cerebrospinal fluid (CSF) and plasma from patients with Alzheimer's disease/senile dementia of Alzheimer type, multi-infarct dementia and aged nondemented individuals were analyzed using isoelectric focusing (IEF) followed by immunofixation and high resolution two-dimensional polyacrylamide gel electrophoresis (PAGE). The IEF profiles of plasma and CSF in patients with dementia were diffuse and lacked oligoclonal bands and were identical to those of nondemented individuals. The investigation of plasma and CSF by two-dimensional PAGE did not reveal any specific protein pattern in the demented individuals compared to the controls.

摘要

使用等电聚焦（IEF），随后进行免疫固定和高分辨率二维聚丙烯酰胺凝胶电泳（PAGE），分析了阿尔茨海默病/阿尔茨海默型老年痴呆症、多发梗死性痴呆患者以及老年非痴呆个体的脑脊液（CSF）和血浆的IgG谱。痴呆患者血浆和脑脊液的IEF谱呈弥散状，缺乏寡克隆带，与非痴呆个体的相同。与对照组相比，二维PAGE对血浆和脑脊液的研究未在痴呆个体中发现任何特异性蛋白质模式。