Wikkelsö C, Andersson M, Andersson R, Blomstrand C
Eur Neurol. 1984;23(4):306-12. doi: 10.1159/000115747.
Cerebrospinal fluid (CSF) and serum samples from 361 consecutive patients were investigated. The protein pattern of each sample was examined by isoelectric focusing (IEF) of unconcentrated CSF followed by silver staining and by agar gel electrophoresis of concentrated CSF stained with amido black. 347 paired samples were compared. The results were the same in 83% of the samples. Discordant results were found in 10% of the samples. and in 7% additional information was achieved by one of the methods. IEF was superior to agar gel electrophoresis for the identification of oligoclonal immune reactions of the central nervous system. Tau globulin increase was seen in a few CSF samples, more often with IEF and then associated with other changes of the pattern. IEF of unconcentrated CSF followed by silver staining only requires 20 microliters CSF, while 3-4 ml CSF is needed for the agar gel electrophoretic method. It was concluded that IEF has several advantages compared to agar gel electrophoresis and is suitable as a routine method in clinical laboratories.
对361例连续患者的脑脊液（CSF）和血清样本进行了研究。通过对未浓缩脑脊液进行等电聚焦（IEF）后银染以及对浓缩脑脊液进行氨基黑染色的琼脂凝胶电泳来检测每个样本的蛋白质图谱。比较了347对样本。83%的样本结果相同。10%的样本结果不一致，7%的样本通过其中一种方法获得了额外信息。在识别中枢神经系统的寡克隆免疫反应方面，IEF优于琼脂凝胶电泳。在少数脑脊液样本中可见tau球蛋白增加，在IEF检测中更常见，且与图谱的其他变化相关。未浓缩脑脊液进行IEF后银染仅需20微升脑脊液，而琼脂凝胶电泳法需要3 - 4毫升脑脊液。得出的结论是，与琼脂凝胶电泳相比，IEF有多个优点，适合作为临床实验室的常规方法。
