Bogdanov Alexey M, Mishin Alexander S, Yampolsky Ilia V, Belousov Vsevolod V, Chudakov Dmitriy M, Subach Fedor V, Verkhusha Vladislav V, Lukyanov Sergey, Lukyanov Konstantin A
Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia.
Nat Chem Biol. 2009 Jul;5(7):459-61. doi: 10.1038/nchembio.174.
Proteins of the green fluorescent protein (GFP) family are well known owing to their unique biochemistry and extensive use as in vivo markers. We discovered that GFPs of diverse origins can act as light-induced electron donors in photochemical reactions with various electron acceptors, including biologically relevant ones. Moreover, via green-to-red GFP photoconversion, this process can be observed in living cells without additional treatment.
绿色荧光蛋白（GFP）家族的蛋白质因其独特的生物化学性质以及作为体内标记物的广泛应用而广为人知。我们发现，不同来源的绿色荧光蛋白在与各种电子受体（包括具有生物学相关性的受体）发生的光化学反应中可作为光诱导电子供体。此外，通过绿色荧光蛋白的绿到红的光转化，这一过程无需额外处理即可在活细胞中观察到。
