评估西班牙东南部药用植物针对核因子-κB和其他促炎介质的潜在抗炎作用。

Assessing medicinal plants from South-Eastern Spain for potential anti-inflammatory effects targeting nuclear factor-Kappa B and other pro-inflammatory mediators.

作者信息

Bremner P, Rivera D, Calzado M A, Obón C, Inocencio C, Beckwith C, Fiebich B L, Muñoz E, Heinrich M

机构信息

The Centre of Pharmacognosy and Phytotherapy, The School of Pharmacy, Univ. London, 29-39 Brunswick Square, London, WC1N 1AX, UK.

出版信息

J Ethnopharmacol. 2009 Jul 15;124(2):295-305. doi: 10.1016/j.jep.2009.04.035. Epub 2009 May 3.

DOI:10.1016/j.jep.2009.04.035

PMID:19397975

Abstract

AIM OF THE STUDY

Identification of plants with anti-inflammatory activity can be successfully based on information gained through knowledge on their traditional use. This is particularly true for biodiversity-rich regions of the world such as the Mediterranean. While such approaches are often single target based, here we used a multitarget, cell-based approach focusing on the pro-inflammatory signaling cascade and especially the NF-kappaB (NF-kappaB) pathway.

MATERIALS AND METHODS

The plants from South-Eastern Spain were chosen on the basis that they were recorded as having a traditional use against an indication related to inflammation. The primary target was the transcription factor NF-kappaB (using a luciferase-based assay in HeLa cells). In addition extracts were tested in vitro for effects on cytokines (IL-6, IL-8, TNF-alpha) or PGE(2) in monocytes and for potential cytotoxic/pro-apoptotic action as well as for their influence on the cell cycle.

RESULTS

Overall, 64 medicinal plant drugs from 61 species were assessed as potential inhibitors of inflammatory mediators to levels of 100-10 microg/ml. Three plants showed the highest level of activity (50 microg/ml) in inhibiting the activation of NF-kappaB in 5.1 cells: Helichrysum stoechas (Asteraceae), Dorycnium pentaphyllum (Fabaceae, s.l.) and Phlomis almeriensis (Lamiaceae). In the tests against the cytokines it was particularly striking to find that a number of species, Bupleurum fruticosum, Chamaespartium tridentatum, Genista ramosissima, Helichrysum stoechas, Mercurialis tomentosa, Ononis ramosissima, Peganum harmala, Picnomon acarna, Retama sphaerocarpa and Santolina viscosa showed extracts that were active at inhibiting TNF-alpha (10 microg/ml).

CONCLUSIONS

Overall, this project has identified a series of species with an activity profile which merits further phytochemical-pharmacological investigation.

摘要

研究目的

基于通过传统用途所获得的信息，能够成功鉴定出具有抗炎活性的植物。对于世界上生物多样性丰富的地区，如地中海地区，尤其如此。虽然此类方法通常基于单一靶点，但在此我们采用了一种基于细胞的多靶点方法，重点关注促炎信号级联反应，特别是核因子κB（NF-κB）途径。

材料与方法

选择西班牙东南部的植物是基于它们有针对与炎症相关病症的传统用途记录。主要靶点是转录因子NF-κB（在HeLa细胞中使用基于荧光素酶的检测方法）。此外，对提取物进行体外测试，以检测其对单核细胞中细胞因子（IL-6、IL-8、TNF-α）或前列腺素E2（PGE2）的影响，以及潜在的细胞毒性/促凋亡作用及其对细胞周期的影响。

结果

总体而言，对来自61个物种的64种药用植物药物作为炎症介质的潜在抑制剂进行了评估，浓度为100 - 10微克/毫升。三种植物在抑制5.1细胞中NF-κB激活方面表现出最高活性水平（50微克/毫升）：蜡菊（菊科）、五叶金雀花（广义豆科）和阿尔梅里亚糙苏（唇形科）。在针对细胞因子的测试中，特别值得注意的是，许多物种，如灌木柴胡、三叉山黑豆、多枝金雀花、蜡菊、绒毛山靛、多枝芒柄花、骆驼蓬、刺芹、圆果骆驼刺和粘性银胶菊的提取物在抑制TNF-α方面具有活性（10微克/毫升）。