De Melo Giany O, Malvar David do C, Vanderlinde Frederico A, Rocha Fabio F, Pires Priscila Andrade, Costa Elson A, de Matos Lécia G, Kaiser Carlos R, Costa Sônia S
Núcleo de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro, 21941-902 Rio de Janeiro, RJ, Brazil.
J Ethnopharmacol. 2009 Jul 15;124(2):228-32. doi: 10.1016/j.jep.2009.04.024. Epub 2009 May 3.
To identify the compounds responsible for the antinociceptive and anti-inflammatory effects previously described for Sedum dendroideum, through bioassay-guided fractionation procedures.
Antinociceptive activity was evaluated through mouse acetic acid-induced writhing model. The anti-inflammatory activity was assessed through croton oil-induced mouse ear oedema and carrageenan-induced peritonitis.
The Sedum dendroideum juice afforded seven known flavonoids identified with basis on NMR data. The oral administration of the major kaempferol glycosides kaempferitrin [1] (17.29 micromol/kg), kaempferol 3-O-beta-glucopyranoside-7-O-alpha-rhamnopyranoside [2] (16.82 micromol/kg), kaempferol 3-O-neohesperidoside-7-O-alpha-rhamnopyranoside [3] (13.50 micromol/kg) or alpha-rhamnoisorobin [5] (23.13 micromol/kg) inhibited by 47.3%, 25.7%, 60.2% and 58.0%, respectively, the acetic acid-induced nociception (indomethacin: 27.95 micromol/kg, p.o.; 68.9%). Flavonoids 1, 2, 3 or 5, at the same doses, reduced by 39.5%, 46.5%, 35.6% and 33.3%, respectively, the croton oil-induced oedema (dexamethasone: 5.09 micromol/kg, s.c.; 83.7%) and impaired leukocyte migration by 42.9%, 46.3%, 50.4% and 49.6%, respectively (dexamethasone: 5.09 micromol/kg, s.c.; 66.1%).
Our findings show that the major kaempferol glycosides may account for the renowned medicinal use of Sedum dendroideum against pain and inflammatory troubles.
通过生物测定导向的分离程序，确定之前所述的垂枝景天具有抗伤害感受和抗炎作用的化合物。
通过小鼠醋酸诱导扭体模型评估抗伤害感受活性。通过巴豆油诱导的小鼠耳肿胀和角叉菜胶诱导的腹膜炎评估抗炎活性。
垂枝景天汁液提供了七种基于核磁共振数据鉴定出的已知黄酮类化合物。口服主要的山奈酚苷山奈酚苷元[1]（17.29微摩尔/千克）、山奈酚3 - O - β - 葡萄糖苷 - 7 - O - α - 鼠李糖苷[2]（16.82微摩尔/千克）、山奈酚3 - O - 新橙皮糖苷 - 7 - O - α - 鼠李糖苷[3]（13.50微摩尔/千克）或α - 鼠李异荭草素[5]（23.13微摩尔/千克）分别抑制醋酸诱导的伤害感受47.3%、25.7%、60.2%和58.0%（吲哚美辛：27.95微摩尔/千克，口服；68.9%）。相同剂量下，黄酮类化合物1、2、3或5分别使巴豆油诱导的肿胀减少39.5%、46.5%、35.6%和33.3%（地塞米松：5.09微摩尔/千克，皮下注射；83.7%），并分别使白细胞迁移受损42.9%、46.3%、50.4%和49.6%（地塞米松：5.09微摩尔/千克，皮下注射；66.1%）。
我们的研究结果表明，主要的山奈酚苷可能是垂枝景天用于治疗疼痛和炎症疾病的著名药用原因。
