Laranjeira Inês Martins, Apolinário Elisabete, Amorim Diana, da Silva Filho Ademar Alves, Dias Alberto Carlos Pires, Pinto-Ribeiro Filipa
Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Campus of Gualtar, 4710-057 Braga, Portugal.
ICVS/3B's-PT Government Associate Laboratory, 4806-909 Guimarães, Portugal.
Foods. 2024 Feb 9;13(4):535. doi: 10.3390/foods13040535.
Osteoarthritis (OA) persistently activates nociceptors, leading to chronic pain, which is often accompanied by the comorbid development of emotional impairments (anxiety and depression), an effect associated with microgliosis. DC (Asteraceae), a Brazilian edible plant, is an important source of active compounds with anti-inflammatory abilities. Thus, we evaluated its ability to reverse OA-induced nociceptive and emotional-like impairments in osteoarthritic ovariectomized female rats using the kaolin/carrageenan (K/C) model. Four weeks after OA induction, mechanical hyperalgesia was confirmed, and the treatment started. Control animals (SHAMs) were treated with phosphate-buffered saline (PBS), while arthritic animals (ARTHs) either received PBS or 50 mg/kg (Bd50) and 100 mg/kg (Bd100), via gavage, daily for five weeks. At the end of the treatment, anxiety-like behavior was assessed using the Open Field Test (OFT), anhedonia was assessed using the Sucrose Preference Test (SPT), and learned helplessness was assessed using the Forced Swimming Test (FST). After occision, microglia were stained with IBA-1 and quantified in brain sections of target areas (prefrontal cortex, amygdala, and periaqueductal grey matter). Treatment with extract reversed OA-induced mechanical hyperalgesia and partly improved depressive-like behavior in OA animals' concomitant to a decrease in the number of M1 microglia. Our findings suggest that extracts can potentially be used in the food industry and for the development of nutraceuticals and functional foods
骨关节炎（OA）持续激活伤害感受器，导致慢性疼痛，且常伴有情绪障碍（焦虑和抑郁）的共病发展，这种影响与小胶质细胞增生有关。DC（菊科）是一种巴西可食用植物，是具有抗炎能力的活性化合物的重要来源。因此，我们使用高岭土/角叉菜胶（K/C）模型评估了其逆转OA诱导的骨关节炎去卵巢雌性大鼠伤害性和类似情绪障碍的能力。在诱导OA四周后，确认出现机械性痛觉过敏并开始治疗。对照动物（假手术组）用磷酸盐缓冲盐水（PBS）治疗，而关节炎动物（关节炎组）通过灌胃每天接受PBS或50mg/kg（Bd50）和100mg/kg（Bd100），持续五周。治疗结束时，使用旷场试验（OFT）评估类似焦虑的行为，使用蔗糖偏好试验（SPT）评估快感缺失，使用强迫游泳试验（FST）评估习得性无助。取材后，用IBA-1对小胶质细胞进行染色，并在目标区域（前额叶皮质、杏仁核和导水管周围灰质）的脑切片中进行定量分析。提取物治疗可逆转OA诱导的机械性痛觉过敏，并部分改善OA动物的抑郁样行为，同时M1小胶质细胞数量减少。我们的研究结果表明，提取物有可能用于食品工业以及营养保健品和功能性食品的开发。
