Ghildyal Reena, Jordan Benjamin, Li Dongsheng, Dagher Hayat, Bardin Phillip G, Gern James E, Jans David A
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia.
J Virol. 2009 Jul;83(14):7349-52. doi: 10.1128/JVI.01748-08. Epub 2009 Apr 29.
The degradation of nuclear pore components and disruption of nucleocytoplasmic trafficking during rhinovirus infection have been attributed to viral 2A protease. Here we show for the first time that rhinovirus 3C protease may also have a role. Specifically, we show that 3C and its precursor, 3CD, can target green fluorescent protein to the nucleus of living cells, leading to degradation of nuclear pore components, and that incubation with recombinant 3C disrupts active and passive nucleocytoplasmic transport in a semi-intact cell nuclear transport system dependent on 3C protease activity. 3C may thus contribute to host cell shutoff in infected cells by localizing in the nucleus and facilitating nuclear pore breakdown.
鼻病毒感染期间核孔成分的降解以及核质运输的破坏一直被认为是由病毒2A蛋白酶所致。在此，我们首次表明鼻病毒3C蛋白酶可能也发挥了作用。具体而言，我们发现3C及其前体3CD可将绿色荧光蛋白靶向活细胞的细胞核，导致核孔成分降解，并且在依赖3C蛋白酶活性的半完整细胞核运输系统中，与重组3C共同孵育会破坏主动和被动的核质运输。因此，3C可能通过定位于细胞核并促进核孔破裂，从而在受感染细胞中导致宿主细胞关闭。
